A single amino acid substitution in PmrB is associated with polymyxin B resistance in clinical isolate ofPseudomonas aeruginosa
| Autor: | Dong H. Kwon, Neethu Abraham |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
DNA
Bacterial medicine.drug_class Polymyxin Molecular Sequence Data Colony Count Microbial Mutation Missense Drug resistance medicine.disease_cause Microbiology chemistry.chemical_compound Minimum inhibitory concentration Bacterial Proteins Drug Resistance Bacterial Genetics medicine Humans Pseudomonas Infections Amino Acid Sequence Molecular Biology Polymyxin B Antibacterial agent Microbial Viability Methionine biology Pseudomonas aeruginosa Genetic Complementation Test Sequence Analysis DNA biology.organism_classification Anti-Bacterial Agents Amino Acid Substitution chemistry Sequence Alignment Transcription Factors Pseudomonadaceae medicine.drug |
| Zdroj: | FEMS Microbiology Letters. 298:249-254 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1111/j.1574-6968.2009.01720.x |
| Popis: | Pseudomonas aeruginosa is a major causative agent of hospital-acquired infections and infections in cystic fibrosis patients. Treatment of P. aeruginosa is complicated by the presence of intrinsic and acquired multidrug-resistant isolates. Polymyxin B has often been used as the last option to treat the multidrug-resistant isolates. However, polymyxin B-resistant clinical isolates have been increasingly reported worldwide. To understand molecular details of polymyxin resistance we characterized polymyxin B-resistant clinical isolate of P. aeruginosa. The clinical isolate grew with 4 microg mL(-1) of polymyxin B while a reference P. aeruginosa PAO1 grew with 0.25 microg mL(-1). Polymyxin B susceptibility was restored (minimal inhibitory concentration from 8 to 0.5 microg mL(-1)) by an intact clone of pmrAB, but not by an intact clone of phoPQ or the cloning vector. DNA sequence analysis of pmrB from the resistant isolate revealed a single nucleotide substitution (T to C) substituted methionine to threonine at position 292 of PmrB. Involvement of this amino acid substitution in polymyxin B resistance was confirmed by complementation of a pmrAB null-mutant strain with the pmrAB containing the amino acid substitution. These results suggest that amino acid substitution in PmrB is one mechanism of polymyxin B resistance in clinical isolates of P. aeruginosa. |
| Databáze: | OpenAIRE |
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