Involvement of CD44 in leukocyte recruitment to the rat testis in experimental autoimmune orchitis
| Autor: | Vanesa Anabella Guazzone, Livia Lustig, Berta Denduchis |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
Embryology Pathology medicine.medical_specialty Receptors Lymphocyte Homing Hyaluronoglucosaminidase Orchitis Biology Active immunization Peripheral blood mononuclear cell Autoimmune Diseases Flow cytometry Rats Sprague-Dawley Andrology Lesion Leukocyte Count Endocrinology Testis medicine Animals Lymph node medicine.diagnostic_test CD44 Obstetrics and Gynecology Cell Biology Flow Cytometry medicine.disease Immunohistochemistry Rats Hyaluronan Receptors medicine.anatomical_structure Reproductive Medicine Apoptosis Acute Disease Leukocytes Mononuclear biology.protein Lymph Nodes medicine.symptom Protein Binding |
| Zdroj: | Reproduction. 129:603-609 |
| ISSN: | 1741-7899 1470-1626 |
| Popis: | Experimental autoimmune orchitis (EAO) is characterized by an interstitial mononuclear cell infiltrate and a severe lesion of the seminiferous tubules with germ cells that undergo apoptosis and sloughing. The aim of this study was to determine the role of CD44 in testicular leukocyte recruitment in EAO. The biological functions of CD44 have been attributed to the generation of a functionally active hyaluronan-binding phenotype. Orchitis was induced in Sprague–Dawley adult rats by active immunization with an emulsion of testicular homogenate and complete Freund’s adjuvant usingBordetella pertussisas co-adjuvant. Control rats (C) injected with saline and adjuvants and normal (N) untreated rats were also studied. CD44 expression was analyzed by flow cytometry in peripheral blood mononuclear cells (PBMC) and lymph node cells isolated from rats at different times after the first immunization. We observed an increase in the mean fluorescence intensity of both samples in the C and experimental (E) groups only after the immunization period. A significant decrease in percentage of CD44 + PBMC and in mean fluorescence intensity was observed in rats with orchitis compared with the C group. Byin vitrohyaluronic acid-binding assay we demonstrated that the percentage of PBMC adhesion was higher in the E group compared with the C and N groups. By immunohistochemistry, we observed a significant increase in the number of CD44 + cells in the testicular interstitium of rats with severe orchitis compared with the N and C groups. These results suggested that the CD44 molecule is involved in the homing of lymphomonocytes into the testes of rats with autoimmune orchitis. |
| Databáze: | OpenAIRE |
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