Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps
| Autor: | Hirschfeld, Josefine, Chicca, Ilaria J., Moonen, Carolyn G.J., White, Phillipa C., Ling, Martin R., Wright, Helen J., Cooper, Paul R., Milward, Mike R., Chapple, Iain L.C., Seymour, Gregory J., Cullinan, Mary P., Heng, Nicholas C.K. |
|---|---|
| Přispěvatelé: | Periodontology, Seymour, Gregory J., Cullinan, Mary P., Heng, Nicholas C.K., Cooper, Paul R. |
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071627792 Oral Biology: Molecular Techniques and Applications, 451-472 STARTPAGE=451;ENDPAGE=472;TITLE=Oral Biology Hirschfeld, J, Chicca, I J, Moonen, C G J, White, P C, Ling, M R, Wright, H J, Cooper, P R, Milward, M R & Chapple, I L C 2023, Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps . in G J Seymour, M P Cullinan, N C K Heng & P R Cooper (eds), Oral Biology : Molecular Techniques and Applications . Methods in Molecular Biology, vol. 2588, Humana Press Inc, pp. 451-472 . https://doi.org/10.1007/978-1-0716-2780-8_27 |
| DOI: | 10.1007/978-1-0716-2780-8_27 |
| Popis: | Following the discovery of neutrophil extracellular traps (NETs) in 2004 by Brinkmann and colleagues, there has been extensive research into the role of NETs in a number of inflammatory diseases, including periodontitis. This chapter describes the current methods for the isolation of peripheral blood neutrophils as well as of oral neutrophils for subsequent NET experiments, including approaches to quantify and visualize NET production, the ability of NETs to entrap and kill bacteria, and the removal of NETs by nuclease-containing plasma. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|