Gestational Diabetes Mellitus Is Associated With Changes in the Concentration and Bioactivity of Placenta-Derived Exosomes in Maternal Circulation Across Gestation

Autor: Gregory E. Rice, Gregory Duncombe, Miharu Kobayashi, Suchismita Sarker, Sebastian E. Illanes, Paula Correa, Katherin Scholz-Romero, Murray D. Mitchell, Emma L. Sweeney, Sherri Longo, Carlos Salomon
Rok vydání: 2015
Předmět:
Male
0301 basic medicine
endocrine system diseases
Placenta
Endocrinology
Diabetes and Metabolism
Exosomes
Pregnancy
Prospective cohort study
Gestational age
Interleukin-10
Gestational diabetes
Uterine Artery
medicine.anatomical_structure
Pregnancy Trimester
Second
Pulsatile Flow
Cytokines
Gestation
Female
Adult
medicine.medical_specialty
Adolescent
Pregnancy Trimester
Third
Enzyme-Linked Immunosorbent Assay
Gestational Age
Exosome
Andrology
Interferon-gamma
Young Adult
03 medical and health sciences
Internal medicine
Diabetes mellitus
Human Umbilical Vein Endothelial Cells
Internal Medicine
medicine
Humans
Inflammation
Interleukin-6
Tumor Necrosis Factor-alpha
business.industry
Interleukin-8
Infant
Newborn
Granulocyte-Macrophage Colony-Stimulating Factor
Ultrasonography
Doppler
Glucose Tolerance Test
medicine.disease
Diabetes
Gestational
030104 developmental biology
Endocrinology
ROC Curve
Case-Control Studies
Interleukin-2
Interleukin-4
business
Zdroj: Diabetes. 65:598-609
ISSN: 1939-327X
0012-1797
Popis: Although there is significant interest in elucidating the role of placenta-derived exosomes (PdEs) during pregnancy, the exosomal profile in pregnancies complicated by gestational diabetes mellitus (GDM) remains to be established. The aim of this study was to compare the gestational-age profile of PdEs in maternal plasma of GDM with normal pregnancies and to determine the effect of exosomes on cytokine release from human umbilical vein endothelial cells. A prospective cohort of patients was sampled at three time points during pregnancy for each patient (i.e., 11–14, 22–24, and 32–36 weeks' gestation). A retrospective stratified study design was used to quantify exosomes present in maternal plasma of normal (n = 13) and GDM (n = 7) pregnancies. Gestational age and pregnancy status were identified as significant factors contributing to variation in plasma exosome concentration (ANOVA, P < 0.05). Post hoc analyses established that PdE concentration increased during gestation in both normal and GDM pregnancies; however, the increase was significantly greater in GDM (∼2.2-fold, ∼1.5-fold, and ∼1.8-fold greater at each gestational age compared with normal pregnancies). Exosomes isolated from GDM pregnancies significantly increased the release of proinflammatory cytokines from endothelial cells. Although the role of exosomes during GDM remains to be fully elucidated, exosome profiles may be of diagnostic utility for screening asymptomatic populations.
Databáze: OpenAIRE
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