Cloning of the mouse gene encoding plasma glutathione peroxidase: organization, sequence and chromosomal localization
| Autor: | Eric Baud, J. P. Dufaure, Norbert B. Ghyselinck, Joël R. Drevet, Véronique Schwaab, Marie-Geneviève Mattei |
|---|---|
| Přispěvatelé: | URA CNRS 1940, Université Blaise Pascal, Aubière |
| Rok vydání: | 1995 |
| Předmět: |
Male
GPX3 Molecular Sequence Data Restriction Mapping Gene Expression Regulatory Sequences Nucleic Acid Biology GPX5 GPX6 Mice Complementary DNA Consensus Sequence Gene expression Genetics Consensus sequence Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Amino Acid Sequence RNA Messenger Cloning Molecular Promoter Regions Genetic Gene Epididymis chemistry.chemical_classification Glutathione Peroxidase Base Sequence Glutathione peroxidase Chromosome Mapping General Medicine Molecular biology Genes chemistry |
| Zdroj: | Gene Gene, 1995, 167 (1-2), pp.25-31. ⟨10.1016/0378-1119(95)00551-x⟩ |
| ISSN: | 0378-1119 1879-0038 |
| DOI: | 10.1016/0378-1119(95)00551-x |
| Popis: | Using a reverse transcription coupled to PCR amplification strategy, with degenerated primers localized in highly conserved domains of known glutathione peroxidase (GPX) proteins, we have generated, from mouse epididymal RNA, a cDNA fragment which was subsequently used to isolate a genomic clone encoding mouse plasma GPX (GPX3). GPX3 is a major enzyme in reducing lipid hydroperoxides and hydrogen peroxide in plasma. We confirm here that the mouse epididymis is a new site of expression of GPX3 and report, together with the sequence, the structural analysis and the chromosomal localization of the mouse GPX3 single-copy gene to chromosome 11. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect