Mucosal immunization with attenuated Shigella flexneri harboring an influenza hemagglutinin DNA vaccine protects mice against a lethal influenza challenge
| Autor: | William R. Jacobs, Luis Martinez-Sobrido, Natalie M. Quanquin, Ana Fernandez-Sesma, Adolfo García-Sastre, Glenn J. Fennelly, William H. Vecino |
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| Rok vydání: | 2004 |
| Předmět: |
T cell
Genetic Vectors Hemagglutinin (influenza) Hemagglutinin Glycoproteins Influenza Virus Biology Vaccines Attenuated medicine.disease_cause Virus Shigella flexneri DNA vaccination Microbiology Mice 03 medical and health sciences 0302 clinical medicine Orthomyxoviridae Infections Immunity Virology Vaccines DNA medicine Animals Shigella 030212 general & internal medicine Immunity Mucosal Administration Intranasal 030304 developmental biology Mice Inbred BALB C 0303 health sciences Base Sequence biochemical phenomena metabolism and nutrition biology.organism_classification 3. Good health medicine.anatomical_structure Immunization Influenza Vaccines biology.protein bacteria Female Plasmids |
| Zdroj: | Virology. 325:192-199 |
| ISSN: | 0042-6822 |
| Popis: | Mucosal surfaces are important for the induction of immunity against influenza virus. In a murine intranasal immunization model, we demonstrated that the attenuated Shigella flexneri Deltaasd strain 15D, carrying a DNA construct encoding the influenza virus hemagglutinin (HA), induces protective immunity against a lethal respiratory challenge with influenza A/WSN/33. Influenza virus-specific IFN-gamma T cells were detected among splenocytes, and anti-HA IgG was detected in serum post-immunization, albeit at low levels. Following influenza virus challenge, an accelerated anti-HA IgA antibody response was detected in bronchoalveolar lavage (BAL) washings from mice vaccinated with attenuated shigella containing the HA construct. These results suggest that S. flexneri Deltaasd strain 15D is a promising vector for mucosal DNA vaccine immunization against influenza virus and other mucosal pathogens. |
| Databáze: | OpenAIRE |
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