Distinguishing between proliferating nodal lymphoid blasts in chronic myelogenous leukemia and non-Hodgkin lymphoma: Report of three cases and detection of a bcr/abl fusion signal by single-cell analysis
| Autor: | Akiko Yashima-Abo, Yusei Aoki, Toru Abo, Chihaya Maesawa, Shugo Kowata, Tomoyuki Masuda, Shigeki Ito, Hitomi Fujiwara, Takashi Satoh, Yoji Ishida |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Male Naphthol AS D Esterase CD3 Complex Fusion Proteins bcr-abl Antigens CD34 Antigens CD7 12E7 Antigen Biology Pathology and Forensic Medicine Antigens CD DNA Nucleotidylexotransferase Leukemia Myelogenous Chronic BCR-ABL Positive hemic and lymphatic diseases medicine Humans In Situ Hybridization Fluorescence Cell Proliferation Peroxidase ABL medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction Lymphoma Non-Hodgkin Naphthol AS breakpoint cluster region General Medicine Middle Aged medicine.disease Immunohistochemistry Lymphoma Blotting Southern Leukemia Cancer research Female Lymph Nodes Cell Adhesion Molecules Chronic myelogenous leukemia K562 cells Fluorescence in situ hybridization |
| Zdroj: | Pathology International. 55:273-279 |
| ISSN: | 1440-1827 1320-5463 |
| DOI: | 10.1111/j.1440-1827.2005.01824.x |
| Popis: | Lymph node biopsies were analyzed from three patients with chronic myelogenous leukemia (CML) showing nodal blast proliferation. Immunohistochemically, the blasts from all three patients had an immature marker profile with a T-blast population (cCD3+, CD4-, CD7+, CD8-, CD99+, terminal deoxynucleotidyl transferase +) and a hematopoietic progenitor cell marker (CD34). In two patients, the blasts also expressed myeloid lineage specificity (naphthol AS-D chloroacetate esterase activity and myeloperoxidase positivity). However, it was difficult to distinguish between blast proliferation in CML and non-Hodgkin lymphoma from these immunohistopathological findings alone. Subsequently, bcr gene rearrangement and bcr/abl mRNA expression were detected by Southern blot and reverse transcription-polymerase chain reaction analysis of the lymph nodes. Fluorescence in situ hybridization (FISH) analysis of lymph node touch smears also disclosed bcr/abl gene fusion signals in the blasts of all patients, confirming that the blasts were derived from Philadelphia chromosome-positive CML. Accurate discrimination between the proliferating nodal blasts of CML and non-Hodgkin lymphoma is essential for determining subsequent therapy. FISH analysis of bcr/abl in single-cell blast preparations is an efficient tool that allows rapid, accurate cytopathological diagnosis of extramedullary blast-phase CML and its discrimination from non-Hodgkin lymphoma. |
| Databáze: | OpenAIRE |
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