Cellular Delivery of Nanoparticles Revealed with Combined Optical and Isotopic Nanoscopy
| Autor: | Christopher R. Anderton, Lizanne G. Nilewski, Nathan C. Gianneschi, Craig Szymanski, Jacquelin K. Kammeyer, Galya Orr, Nia C. Bell, Joseph P. Patterson, Dehong Hu, Anthony M. Rush, Zihua Zhu, Maria T. Proetto, Stephen B. Howell, James E. Evans |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Organoplatinum Compounds
Pyridines Polymers Endocytic cycle General Physics and Astronomy Nanoparticle 02 engineering and technology 01 natural sciences Nanomaterials Coordination Complexes Nanotechnology NanoSIMS General Materials Science drug-loaded nanoparticles Drug Carriers Optical Imaging General Engineering SIM 021001 nanoscience & nanotechnology Fluorescence Endocytosis cytotoxicity fluorescence 0210 nano-technology Intracellular Biotechnology platinum(II) complexes Materials science Cell Survival Surface Properties 1.1 Normal biological development and functioning Antineoplastic Agents Bioengineering 010402 general chemistry Article Underpinning research Amphiphile Humans Particle Size Nanoscience & Nanotechnology technology industry and agriculture 0104 chemical sciences Drug Liberation A549 Cells Hela Cells drug delivery Nanoparticles Generic health relevance Nanocarriers Nanoscale secondary ion mass spectrometry HeLa Cells |
| Zdroj: | Proetto, MT; Anderton, CR; Hu, D; Szymanski, CJ; Zhu, Z; Patterson, JP; et al.(2016). Cellular Delivery of Nanoparticles Revealed with Combined Optical and Isotopic Nanoscopy. ACS NANO, 10(4), 4046-4054. doi: 10.1021/acsnano.5b06477. UC San Diego: Retrieved from: http://www.escholarship.org/uc/item/4bc6433k ACS nano, vol 10, iss 4 ACS Nano |
| DOI: | 10.1021/acsnano.5b06477. |
| Popis: | Direct polymerization of an oxaliplatin analogue was used to reproducibly generate amphiphiles in one pot, which consistently and spontaneously self-assemble into well-defined nanoparticles (NPs). Despite inefficient drug leakage in cell-free assays, the NPs were observed to be as cytotoxic as free oxaliplatin in cell culture experiments. We investigated this phenomenon by super-resolution fluorescence structured illumination microscopy (SIM) and nanoscale secondary ion mass spectrometry (NanoSIMS). In combination, these techniques revealed NPs are taken up via endocytic pathways before intracellular release of their cytotoxic cargo. As with other drug-carrying nanomaterials, these systems have potential as cellular delivery vehicles. However, high-resolution methods to track nanocarriers and their cargo at the micro- and nanoscale have been underutilized in general, limiting our understanding of their interactions with cells and tissues. We contend this type of combined optical and isotopic imaging strategy represents a powerful and potentially generalizable methodology for cellular tracking of nanocarriers and their cargo. |
| Databáze: | OpenAIRE |
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