Innate cell-mediated cytotoxicity of CD8+ T cells against the protozoan parasite Ichthyophthirius multifiliis in the ginbuna crucian carp Carassius auratus langsdorfii
| Autor: | Koumei Shiota, Masaki Sukeda, Tomonori Somamoto, Miki Nakao, Takahiro Nagasawa, Masakazu Kondo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Innate immune system Ichthyophthirius multifiliis biology Cytotoxic activity ginbuna crucian carp Immunology Acquired immune system biology.organism_classification CD8+ T cells Molecular biology I. multifiliis extracellular parasites 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Perforin Crucian carp biology.protein Cytotoxic T cell Cytotoxicity CD8 030215 immunology Developmental Biology |
| Zdroj: | Developmental & Comparative Immunology. 115(103886) |
| ISSN: | 0145-305X |
| Popis: | Cytotoxic T cells are known to have the ability to kill microbe-infected host cells, which makes them essential in the adaptive immunity processes of various vertebrates. In this study, we demonstrated innate cell-mediated cytotoxicity of CD8+ T cells against protozoan parasites found in the ginbuna crucian carp. When isolated effector cells such as CD8+, CD4+ (CD4-1+), or CD8- CD4- (double-negative, DN), from naive ginbuna crucian carp were co-incubated with target parasites (Ichthyophthirius multifiliis), CD8+ cells from the kidney and gill showed the highest cytotoxic activity. On the other hand, DN cells, which include macrophages and CD4- CD8- lymphocytes, showed the lowest cytotoxic activity against I. multifiliis. Additionally, the cytotoxic activity of CD8+ cells was found to significantly decrease in the presence of a membrane separating the effector cells from I. multifiliis. Furthermore, the serine protease inhibitor 3,4-dichloroisocoumarin and perforin inhibitor concanamycin A significantly inhibited the cytotoxic activity of CD8+ cells. These results demonstrate that CD8+ T cells of ginbuna crucian carp can kill extracellular parasites in a contact-dependent manner via serine proteases and perforin. Therefore, we conclude that CD8+ T cells play an essential role in anti-parasite innate immunity of teleost fish. |
| Databáze: | OpenAIRE |
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