A xylogalactofucan from the brown seaweed Spatoglossum schröederi stimulates the synthesis of an antithrombotic heparan sulfate from endothelial cells
| Autor: | Leandro Silva da Costa, Luana Cristina Lins de Medeiros Bezerra, Hugo Alexandre Oliveira Rocha, Celina Maria Pinto Guerra, Luiz Diz de Abreu, Edda Lisboa Leite, Helena B. Nader, Ivan Rui Lopes Albuquerque |
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| Rok vydání: | 2005 |
| Předmět: |
Ion chromatography
Pharmaceutical Science Xylose High-performance liquid chromatography Fucose Analytical Chemistry chemistry.chemical_compound Drug Discovery Dictyotaceae Humans Pharmacology biology Plant Extracts Organic Chemistry Thrombin Anticoagulants Endothelial Cells Heparan sulfate biology.organism_classification Seaweed Complementary and alternative medicine Biochemistry chemistry Sephadex Galactose Molecular Medicine Heparitin Sulfate Phytotherapy |
| Zdroj: | Planta medica. 71(4) |
| ISSN: | 0032-0943 |
| Popis: | The brown seaweed Spatoglossum schroederi (Dictyotaceae) contains three main fucans (fucans A, B and C) with different mobility in electrophoresis. The fucan with highest mobility (fucan C) was precipitated with 2.0 volumes of acetone, purified using a combination of ion exchange chromatography and electrophoresis. It showed an MW of 24 kDa determined by HPLC and Sephadex G-75 chromatography and migrates as a single band in three distinct electrophoretic systems. This fucan contains fucose, xylose, galactose and sulfate in a molar ratio 1 : 0.6 : 2:2.3. The fucan has neither anticoagulant (from 10 to 100 microg) nor hemorrhagic activities (100 microg/mL). In addition, fucan C is neither cytotoxic nor cytostatic. However, fucan C (100 microg/mL) stimulated the synthesis of an antithrombotic heparan sulfate from endothelial cells of rabbit aorta. The results suggest that fucan C might be used as an antithrombotic therapeutic compound. |
| Databáze: | OpenAIRE |
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