Direct chiral separation of troglitazone stereoisomers using reversed-phase high-performance liquid chromatography
| Autor: | Takao Yoshioka, Shinya Tsutsumi, Akira Takemura, Nobuyuki Suzuki, Takao Kawasaki, Akifumi Miyamoto |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Chemistry
Pharmaceutical Clinical Biochemistry Thiazolidine Analytical chemistry Pharmaceutical Science High-performance liquid chromatography Dosage form Analytical Chemistry chemistry.chemical_compound Troglitazone Drug Discovery medicine Chromans Spectroscopy Chromatography High Pressure Liquid Response factor Chromatography Reproducibility of Results Stereoisomerism Reversed-phase chromatography Repeatability Thiazoles chemistry Thiazolidinediones Methanol medicine.drug |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 30(3) |
| ISSN: | 0731-7085 |
| Popis: | A simple HPLC method for the direct chiral separation of troglitazone stereoisomers was developed. The separation was performed on a reversed-phase cellulose-derivertized chiral column (Chiralcel OJ-R) using a mobile phase consisting of methanol–acetic acid (1000:1, v/v) at a flow rate of 0.5 ml/min. The peak areas of stereoisomers separated from 0.13 to 0.75 mg/ml of troglitazone had good linearity, with correlation coefficients >0.999 in the reversed-phase mode. The repeatability of the ratios of stereoisomers isolated from 0.5 mg/ml of troglitazone had a relative standard deviation of 0.1–0.2%. The relative sensitivities of the four isomers at UV 285 nm were similar, as each response factor was within the range of 0.99–1.01. Troglitazone racemized at the chiral center of the thiazolidine ring in methanol solution, but was found to be stable for 24 h in methanol–acetic acid (1000:1, v/v). This method was applied to the stereoisomeric analysis of troglitazone in pharmaceutical formulations and used to evaluate the constancy of the stereoisomer ratio in the manufacturing process and stability testing. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect