Previous exposure to glucose enhances somatostatin secretion from the isolated perfused rat pancreas
| Autor: | Valdemar Grill, M. Rundfeldt, S. Efendić |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Male
medicine.medical_specialty Arginine Somatostatin secretion Endocrinology Diabetes and Metabolism medicine.medical_treatment Stimulation In Vitro Techniques Biology Islets of Langerhans chemistry.chemical_compound Internal medicine Insulin Secretion Internal Medicine medicine Animals Insulin Glucose homeostasis Glucagon secretion Fasting Glucagon Rats Perfusion Kinetics Glucose Endocrinology Somatostatin L-Glucose chemistry |
| Zdroj: | Diabetologia. 20:495-500 |
| ISSN: | 1432-0428 0012-186X |
| DOI: | 10.1007/bf00253414 |
| Popis: | Previous exposure to glucose enhances insulin and depresses glucagon secretion by the pancreas. We have investigated whether secretion of somatostatin is also influenced by a glucose priming effect. In perfused rat pancreas from 36 h fasted rats a 5 min pulse of arginine (8 mmol/l) rapidly elicited a peak of somatostatin release. A similar somatostatin response was evoked by a second, identical, pulse of arginine after perfusion with "basal" glucose (3.9 mmol/l) for 45 min. On the other hand when 27.7 mmol/l D-glucose, was administered for 20 min between arginine pulses, there was significant stimulation of somatostatin secretion. When arginine was re-introduced 15 min after the cessation of the pulse of elevated glucose the magnitude of the arginine-induced peak (min 0-2 of stimulation) was increased from 16.2 +/- 4.1 to 33.1 +/- 4.7 pg/2 min, p less than 0.01, relative to the first stimulation with arginine. None of these effects of glucose could be reproduced by D-galactose. The somatostatin response to arginine was higher in pancreata from fed than from 36 h fasted animals as was also basal release (22.8 +/- 5.0 vs 9.0 +/- 2.0 pg/min). In the fed state the response to the second pulse of arginine was however reduced by 50% after perfusion with "basal" glucose. This decrease in responsiveness was counteracted by perfusion with 27.7 mmol/l glucose for 20 min between the arginine pulses. It is concluded that previous exposure to an elevated concentration of glucose enhanced D-cell responsiveness to arginine in the fasted as well as the fed state. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink