Development of a SCAR (sequence-characterised amplified region) marker for acid resistance-related gene in Lactobacillus plantarum
| Autor: | Shi‑Ling Yang, Shu‑Fen Tian, Ling He, Kai Li, Shu‑Wen Liu |
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| Rok vydání: | 2014 |
| Předmět: |
Genetic Markers
Molecular Sequence Data Acid resistance Biology Microbiology chemistry.chemical_compound Stress Physiological Drug Resistance Bacterial Malolactic fermentation Related gene Gene Sequence (medicine) Genetics Base Sequence Gene Amplification food and beverages General Medicine biology.organism_classification Molecular biology chemistry Genetic marker Genes Bacterial Molecular Medicine Acids DNA Lactobacillus plantarum |
| Zdroj: | Extremophiles : life under extreme conditions. 19(2) |
| ISSN: | 1433-4909 |
| Popis: | A sequence characterised amplified region marker was developed to determine an acid resistance-related gene in Lactobacillus plantarum. A random amplified polymorphic DNA marker named S116-680 was reported to be closely related to the acid resistance of the strains. The DNA band corresponding to this marker was cloned and sequenced with the induction of specific designed PCR primers. The results of PCR test helped to amplify a clear specific band of 680 bp in the tested acid-resistant strains. S116-680 marker would be useful to explore the acid-resistant mechanism of L. plantarum and to screen desirable malolactic fermentation strains. |
| Databáze: | OpenAIRE |
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