Human complement factor B: functional properties of a recombinant zymogen of the alternative activation pathway convertase
| Autor: | Wilhelm J. Schwaeble, Keith Whaley, Tobias Sokolowski, Karl-Hermann Meyer zum Büschenfelde, Cornelia Estaller, B. Lüttig, Elisabeth H. Weiss, Wolfgang Dippold |
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| Rok vydání: | 1993 |
| Předmět: |
Immunology
Complement Pathway Alternative Immunoblotting Molecular Sequence Data Enzyme-Linked Immunosorbent Assay Complement C3-C5 Convertases Biology Transfection Complement factor B Complement Hemolytic Activity Assay law.invention Cell Line law Immunology and Allergy Animals Humans Enzyme Precursors Complement component 3 Base Sequence Hematology DNA Haemolysis Recombinant Proteins Complement system Biochemistry Factor H Recombinant DNA Alternative complement pathway CFHR5 Complement Factor B |
| Zdroj: | Immunobiology. 188(3) |
| ISSN: | 0171-2985 |
| Popis: | The human complement factor B is a centrally important component of the alternative pathway activation of the complement system. Here we report the isolation, characterization and eukaryotic expression of the first full length cDNA transcript for human factor B. In a factor B dependent haemolysis assay, the recombinant human factor B generated by transient COS cell transfection was shown to reconstitute haemolytic activity of factor B depleted human serum. To study the biological activities assigned to factor B, the availability of recombinant polypeptides representing definite portions of the human factor B molecule is desirable. |
| Databáze: | OpenAIRE |
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