Oncogenic Role of MicroRNA-30b-5p in Glioblastoma Through Targeting Proline-Rich Transmembrane Protein 2
| Autor: | Longbo Zhang, Yujie Ma, Jun-Xiu Guo, Zhi-Xiong Lin, Zhong-Jun Li |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research Nerve Tissue Proteins Biology Article 03 medical and health sciences Proline-Rich Transmembrane Protein 2 0302 clinical medicine Downregulation and upregulation Cell Movement RNA interference Cell Line Tumor microRNA Humans U87 3' Untranslated Regions neoplasms Oncogene Cell Proliferation Regulation of gene expression Gene knockdown Proline-rich transmembrane protein 2 (PRRT2) Membrane Proteins MicroRNA Tumor suppressor Oncogenes General Medicine nervous system diseases Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology Oncology Cell culture Gene Knockdown Techniques 030220 oncology & carcinogenesis Cancer research RNA Interference Glioblastoma |
| Zdroj: | Oncology Research |
| ISSN: | 0965-0407 |
| DOI: | 10.3727/096504017x14944585873659 |
| Popis: | MicroRNAs (miRs) have been found to play promoting or suppressive roles in different human cancers. However, the exact regulatory mechanism of miR-30b in glioblastoma remains unknown. Here we have shown that the expression of miR-30b is significantly increased in glioblastoma tissues and cell lines. Moreover, a high expression of miR-30b is significantly associated with a shorter survival time for glioblastoma patients. Knockdown of miR-30b caused a significant reduction in the proliferation, migration, and invasion of U87 and A172 cells. Proline-rich transmembrane protein 2 (PRRT2) was further identified as a novel target gene of miR-30b, and its protein expression is negatively regulated by miR-30b in U87 and A172 cells. Furthermore, PRRT2 is significantly downregulated in glioblastoma tissues and cell lines, and we found an inverse correlation between miR-30b and PRRT2 expression in glioblastoma tissues. In addition, inhibition of PRRT2 reversed the suppressive effect of miR-30b downregulation on the malignant phenotypes of U87 and A172 cells. Accordingly, we demonstrated that miR-30b promotes glioblastoma cell proliferation, migration, and invasion via targeting PRRT2. Therefore, miR-30b may be used as a promising therapeutic target for glioblastoma. |
| Databáze: | OpenAIRE |
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