Validation of an Enzyme-Linked Immunosorbent Assay for Detecting Sulfonamides in Feed Resources
| Autor: | J. Guiteras, R. Companyó, Maria-Pilar Marco, Javier Adrian, Vanesa Jiménez |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Detection limit
Feed analysis Sulfonamides Chromatography medicine.diagnostic_test Chemistry Extraction (chemistry) Enzyme-Linked Immunosorbent Assay General Chemistry Elisa assay Animal Feed Sulfadiazine Limit of Detection Immunoassay Screening method medicine General Agricultural and Biological Sciences medicine.drug |
| Zdroj: | Journal of Agricultural and Food Chemistry. 58:7526-7531 |
| ISSN: | 1520-5118 0021-8561 |
| Popis: | An enzyme-linked immunosorbent assay (ELISA) to screen sulfadiazine and sulfamethazine residues in feeds has been developed and validated according to Commission Decision 2002/657/EC criteria. Sulfonamides are easily extracted with a 95:5 acetonitrile/water mixture, obtaining recoveries between 80 and 100%. Accuracy, precision, selectivity, robustness, limit of detection (LOD), and detection capability (CCbeta) of the assay have been assessed during the validation process. LOD values in pig feed samples were 0.2 microg/g for sulfadiazine and 0.04 microg/g for sulfamethazine without any sample treatment other than extraction, dilution with the assay buffer, and filtering of the resulting solution. Furthermore, a new strategy for the determination of CCbeta in an ELISA screening method is proposed; this gave CCbeta values of 0.8 microg/g for sulfadiazine and 0.1 microg/g for sulfamethazine. Besides sulfadiazine and sulfamethazine, other sulfonamides can be detected with this immunoassay; this has been verified calculating their LOD values and cross-reactivities. Finally, real feed samples were analyzed with the ELISA methodology and a previously developed liquid chromatography (LC) method, and results confirmed the utility of this new immunoassay for screening purposes. |
| Databáze: | OpenAIRE |
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