Immunologic timeline of Ebola virus disease and recovery in humans
| Autor: | Stuart T. Nichol, Han Chen, David R. Mcllwain, Nilanjan Mukherjee, Garry P. Nolan, Rama Akondy, Anita K. McElroy, Christina F. Spiropoulou, Aneesh K. Mehta, Zach Bjornson-Hooper |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Myeloid T cell medicine.disease_cause 03 medical and health sciences 0302 clinical medicine Immune system medicine Humans Ebola virus biology Convalescence General Medicine Monocyte proliferation Hemorrhagic Fever Ebola Viral Load Ebolavirus Haematopoiesis 030104 developmental biology medicine.anatomical_structure Ki-67 Antigen 030220 oncology & carcinogenesis Immunology biology.protein Leukocytes Mononuclear Antibody Viral load Research Article |
| Zdroj: | JCI Insight |
| Popis: | A complete understanding of human immune responses to Ebola virus infection is limited by the availability of specimens and the requirement for biosafety level 4 (BSL-4) containment. In an effort to bridge this gap, we evaluated cryopreserved PBMCs from 4 patients who survived Ebola virus disease (EVD) using an established mass cytometry antibody panel to characterize various cell populations during both the acute and convalescent phases. Acute loss of nonclassical monocytes and myeloid DCs, especially CD1c(+) DCs, was noted. Classical monocyte proliferation and CD38 upregulation on plasmacytoid DCs coincided with declining viral load. Unsupervised analysis of cell abundance demonstrated acute declines in monocytic, NK, and T cell populations, but some populations, many of myeloid origin, increased in abundance during the acute phase, suggesting emergency hematopoiesis. Despite cell losses during the acute phase, upregulation of Ki-67 correlated with recovery of cell populations over time. These data provide insights into the human immune response during EVD. |
| Databáze: | OpenAIRE |
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