Simplified procedure for measurement of serum dehydroepiandrosterone and its sulfate with gas chromatography–ion trap mass spectrometry and selected reaction monitoring
| Autor: | Patricia D. Kroboth, Michael A. Zemaitis |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Quality Control
endocrine system Chromatography Dehydroepiandrosterone Sulfate Methanol Selected reaction monitoring Reproducibility of Results Dehydroepiandrosterone General Chemistry Mass spectrometry Sensitivity and Specificity Gas Chromatography-Mass Spectrometry chemistry.chemical_compound Dehydroepiandrosterone sulfate chemistry polycyclic compounds Humans Gas chromatography Ion trap Gas chromatography–mass spectrometry skin and connective tissue diseases Derivatization human activities hormones hormone substitutes and hormone antagonists |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 716:19-26 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/s0378-4347(98)00336-3 |
| Popis: | Dehydroepiandrosterone (DHEA) and dehydroepiandrosterone sulfate (DHEAS) are endogenous steroids that have recently been widely publicized as potential treatments for many disorders. This paper describes a gas chromatographic-ion trap mass spectrophotometric assay with selected reaction monitoring for measurement of DHEA and DHEAS levels. The hormones and internal standard (5-androsten-3beta-ol-16-one methyl ester) are extracted from serum with Oasis solid-phase extraction tubes. The extracted steroids are dissolved in methanol and injected into a Finnigan GCQ ion trap mass spectrometer. In the selected reaction mode, both DHEA and DHEAS can be identified and quantified in a single injection. No derivatization or expensive deuterated internal standards are required. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|