A laminated, flex structure for electronic transport and hybridization of DNA
| Autor: | Paul D. Swanson, Trevor Young, Michael Krihak, Anita H. Forster, Donald E. Ackley |
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| Rok vydání: | 2001 |
| Předmět: |
Materials science
Base Sequence Oligonucleotide Biomedical Engineering Biophysics Nucleic Acid Hybridization Nanotechnology Biosensing Techniques Equipment Design General Medicine Flexible electronics Electron Transport Electrophoresis Electrode Electrochemistry Miniaturization DNA Probes Layer (electronics) Flip chip Polyimide Oligonucleotide Array Sequence Analysis Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 16:187-194 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/s0956-5663(01)00121-x |
| Popis: | We have developed the first prototypes of a three-dimensional, electrophoretically driven microlaboratory for the analysis of proteins and DNA. By selecting the appropriate spacing and geometrical configuration, oligonucleotides were transported, in a controlled, rapid fashion, by electrophoresis in free-space. Transport efficiencies over 2 mm distances exceeded 70%. Electrodes of similar design were combined with an electronically addressed DNA hybridization chip to form a fully electrophoretic microlaboratory. In this instance, gold-plated copper electrodes were patterned on a 2 mil thick polyimide substrate. This polyimide layer was stiffened with 20 mil of polyimide to provide support for flip-chip bonding of our standard 100-site Nanochip. This composite structure illustrated three-dimensional transport of target oligonucleotides, through a via in the polyimide, along a series of electrodes and onto the diagnostic chip. Upon reaching the diagnostic chip, electronic hybridization was performed for a competitive reverse dot blot assay. The electronic assay showed a specific to nonspecific ratio in excess of 20:1. These results suggested that this type of structure might be of practical consequence with the development of a microlaboratory for biowarfare applications. |
| Databáze: | OpenAIRE |
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