Identification and Characterization of a Novel Human Cortistatin-like Peptide
| Autor: | Shoji Fukusumi, Shuji Hinuma, Masahiko Fujino, Junko Sakamoto, Masaomi Miyamoto, Kazuaki Kitano, Shiro Takekawa, Chieko Kitada, Hideki Kizawa |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Male
DNA Complementary Molecular Sequence Data Biophysics Peptide Biology Hippocampus Biochemistry Homology (biology) chemistry.chemical_compound Complementary DNA Cyclic AMP Animals Humans Amino Acid Sequence RNA Messenger Receptors Somatostatin Rats Wistar Molecular Biology Cerebral Cortex chemistry.chemical_classification Expressed sequence tag Forskolin Base Sequence Sequence Homology Amino Acid Electromyography cDNA library Somatostatin receptor Neuropeptides Electroencephalography Cell Biology Molecular biology Rats Amino acid chemistry Carrier Proteins Protein Binding |
| Zdroj: | Biochemical and Biophysical Research Communications. 232:157-163 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1997.6252 |
| Popis: | Expressed sequence tags (ESTs) that showed significant homology to rat cortistatin (CST) were found in a human fetal brain cDNA library. A protein coded by the cDNA showed 55% identity to rat preprocortistatin in amino acid. Similarly in the generation of mature peptides from rat preprocortistatin, it was expected that cleavage at dibasic amino acids in the C-terminal portion of the coded protein might produce at least two different sizes of mature peptides with 29 and 17 amino acid residues, respectively. We chemically synthesized the predicted mature peptide with 17 amino acid residues (hCS-17) and examined its biological activities. It bound to all human somatostatin receptor (SSTR) subtypes in almost the same manner as rat CST-14. It also inhibited cAMP production induced by forskolin through SSTRs. Administration of hCS-17 to the cerebral ventricle showed flattening of cortical and hippocampal electroencephalograms in rats. These results indicate that a bioactive peptide encoded by the cDNA is a human counterpart corresponding to rat CST. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect