Molecular characterization of some selected persimmon genotypes and cultivars by srap and ssr markers

Autor: Mustafa Kaplankiran, Mustafa Unlu, Ercan Yildiz, Sezai Ercisli, Celil Toplu, Hasan Pinar, Sedat Serçe
Přispěvatelé: Uşak Üniversitesi
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Genetika, Vol 49, Iss 2, Pp 693-704 (2017)
ISSN: 1820-6069
0534-0012
Popis: In this study, SRAP and SSR markers were employed to determine genetic relationships among 42 persimmon genotypes (Diospyros kaki Thunb) obtained from Hatay province and 3 persimmon cultivars, 2 of which belong to Diospyros kaki Thunb and one belongs to Diospyros oleifera Cheng. Genetic relationships were determined by using a total of 29 molecular DNA primers (SRAP and SSR). Of these primers, 21 SRAP primer combinations produced a total of 107 bands and 77.6% of them were polymorphic; 8 SSR primers produced 26 polymorphic bands with an average polymorphism ratio of 84.6%. The SRAP and SSR markers produced 4.6 bands as average and the number of bands produced per marker was calculated as 3.6. The lowest similarity was observed between MK-113 (Diospyros oleifera Cheng) and the other genotypes all belongs to Diospyros kaki Thunb (with similarity ratios of 0.41-0.69 for SRAP primers, between 0.25-0.67 for SSR primers). The genotypes/cultivars belongs to Diospyros kaki had similarity ratio between 0.98-1.00 according to SRAP and SSR markers. This synonym or similarity could be results of clonal propagation rather than autogamy.
Databáze: OpenAIRE