| Autor: |
Masaracchia, Caterina, Hnida, Marilena, Gerhardt, Ellen, Fonseca, Tomás Lopes Da, Villar-Pique, Anna, Branco, Tiago, Stahlberg, Markus, Camin Dean, Fernández, Claudio, Milosevic, Ira, Outeiro, Tiago |
| Rok vydání: |
2018 |
| DOI: |
10.6084/m9.figshare.6968762.v1 |
| Popis: |
Figure S3. Dyngo blocks whereas PitStop enhances the internalization of aSyn. (A) ICC of H4 cells treated with 1 μM aSyn monomers and with vehicle, PitStop 30 μM or Dyngo 50 μM. Both PitStop and Dyngo are inhibitors of the endocytic processes. (B) Quantification of the aSyn mean fluorescence intensity in the three conditions. Scale bar: 30 μm. (C) Immunoblotting of H4 cells treated with different concentrations of PitStop and Dyngo. (D) Quantification of the immunoblot. Dotted bars refer to the band corresponding to aSyn dimers (aSyn**), and clear bars refer to aSyn monomers (aSyn*). Statistical tests were performed using one-way-analysis of variance (ANOVA), with repeated-measures for grouped analysis, followed by Tukey’s post-hoc tests. Data were expressed as mean ± SEM and a 0.5% general significance level was defined, with significance levels as follows: *: p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
