Serotonin excites fast-spiking interneurons in the striatum
| Autor: | Enrico Bracci, Craig Blomeley |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Male
Serotonin Ketanserin Patch-Clamp Techniques Action Potentials RS-102221 Striatum Tetrodotoxin Biology Synaptic Mechanisms 5-hydroxytryptamine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Interneurons Neuromodulation medicine Receptor Serotonin 5-HT2C Animals rat Rats Wistar Neurotransmitter 030304 developmental biology 0303 health sciences General Neuroscience Corpus Striatum Rats GABAergic interneuron medicine.anatomical_structure chemistry Barium basal ganglia Excitatory postsynaptic potential Female Serotonin Antagonists Raphe nuclei Neuroscience 030217 neurology & neurosurgery medicine.drug Sodium Channel Blockers |
| Zdroj: | The European Journal of Neuroscience |
| ISSN: | 1460-9568 0953-816X |
| Popis: | Fast-spiking interneurons (FSIs) control the output of the striatum by mediating feed-forward GABAergic inhibition of projection neurons. Their neuromodulation can therefore critically affect the operation of the basal ganglia. We studied the effects of 5-hydroxytryptamine (5-HT, serotonin), a neurotransmitter released in the striatum by fibres originating in the raphe nuclei, on FSIs recorded with whole-cell techniques in rat brain slices. Bath application of serotonin (30 microm) elicited slow, reversible depolarizations (9 +/- 3 mV) in 37/46 FSIs. Similar effects were observed using conventional whole-cell and gramicidin perforated-patch techniques. The serotonin effects persisted in the presence of tetrodotoxin and were mediated by 5-HT(2C) receptors, as they were reversed by the 5-HT(2) receptor antagonist ketanserin and by the selective 5-HT(2C) receptor antagonist RS 102221. Serotonin-induced depolarizations were not accompanied by a significant change in FSI input resistance. Serotonin caused the appearance of spontaneous firing in a minority (5/35) of responsive FSIs, whereas it strongly increased FSI excitability in each of the remaining responsive FSIs, significantly decreasing the latency of the first spike evoked by a current step and increasing spike frequency. Voltage-clamp experiments revealed that serotonin suppressed a current that reversed around -100 mV and displayed a marked inward rectification, a finding that explains the lack of effects of serotonin on input resistance. Consistently, the effects of serotonin were completely occluded by low concentrations of extracellular barium, which selectively blocks Kir2 channels. We concluded that the excitatory effects of serotonin on FSIs were mediated by 5-HT(2C) receptors and involved suppression of an inwardly rectifying K(+) current. |
| Databáze: | OpenAIRE |
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