Somatomedin inhibitors from human serum produce abnormalities in mouse embryos in culture
| Autor: | Marc V. L. Du Caju, Wayne Balkan, T. W. Sadler, Raoul Rooman, Lawrence S. Phillips, Steven Goldstein, Amy Hurst‐Evans |
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| Rok vydání: | 1988 |
| Předmět: |
Embryology
medicine.medical_specialty animal structures food.ingredient Health Toxicology and Mutagenesis Biology Peptide hormone Toxicology Mice food Organ Culture Techniques Somatomedins Yolk Internal medicine medicine Animals Humans Neural Tube Defects Uremia Mice Inbred ICR Mutagenicity Tests Embryo culture Embryo medicine.disease Embryo Mammalian Somatomedin Teratology Molecular Weight Endocrinology embryonic structures Azotemia Developmental Biology |
| Zdroj: | Teratology. 38(1) |
| ISSN: | 0040-3709 |
| Popis: | Two human serum fractions, one from normal individuals (Mr 1,150-1,310 daltons) and the other (Mr 800-1,100 daltons) from patients suffering with uremia (renal failure, azotemia), were added to the medium used to grow embryos in whole-embryo culture (WEC) beginning at the 3-5 (day 9) or 18-21 (day 10) somite stage. Both of these fractions possessed somatomedin (insulin-like growth factor) inhibitory activity. Day 9 embryos exposed to either of the serum fractions for 24 hr exhibited incomplete rotation and neural tube closure defects and were smaller than control embryos (decreased total protein content). Developmental abnormalities induced in day 10 embryos following 24 hr in culture included a marked decrease in expansion of the brain regions, hypoplasia of the first two branchial arches, and decreased amounts of total protein compared to controls. The visceral yolk sacs (VYSs) of somatomedin inhibitor (SI)-exposed conceptuses were opaque, and those from day 10 conceptuses contained significantly more protein than controls. Morphologically, the VYS endoderm cells from SI-exposed embryos contained a much higher density of "vacuoles" than controls. These results mimic those produced by exposure of conceptuses to an SI of Mr800-1,100 obtained from the serum of diabetic rats and suggest that similar substances and mechanisms are involved. |
| Databáze: | OpenAIRE |
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