A Novel Tetrodotoxin-sensitive, Voltage-gated Sodium Channel Expressed in Rat and Human Dorsal Root Ganglia
| Autor: | Elda Tzoumaka, Richard M. Eglen, Douglas Kenneth Rabert, Hardy W. Chan, Sanja D. Novakovic, Stephen Gregory Delgado, Ronald C. Herman, Gregory R. Stewart, Ping Sze, Linda Marie Fish, Mariola Ilnicka, Kimberley Wong, Lyn B. Jakeman, Bruce D. Koch, Lakshmi Sangameswaran, John C. Hunter |
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| Rok vydání: | 1997 |
| Předmět: |
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Complementary Transcription Genetic Macromolecular Substances Molecular Sequence Data Xenopus Neuropeptide Tetrodotoxin Biology Polymerase Chain Reaction Biochemistry Sodium Channels Membrane Potentials Xenopus laevis chemistry.chemical_compound Dorsal root ganglion Ganglia Spinal medicine Paroxysmal extreme pain disorder Animals Humans Amino Acid Sequence RNA Messenger Cloning Molecular Molecular Biology Peptide sequence Neurons Messenger RNA Sodium channel NAV1.7 Voltage-Gated Sodium Channel Neuropeptides Cell Biology Anatomy biology.organism_classification medicine.disease Rats Cell biology medicine.anatomical_structure chemistry Organ Specificity Oocytes |
| Zdroj: | Journal of Biological Chemistry. 272:14805-14809 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.272.23.14805 |
| Popis: | Dorsal root ganglion neurons express a wide repertoire of sodium channels with different properties. Here, we report the cloning from rat, dorsal root ganglia (DRG), cellular expression, and functional analysis of a novel tetrodotoxin-sensitive peripheral sodium channel (PN), PN1. PN1 mRNA is expressed in many different tissues. Within the rat DRG, both the mRNA and PN1-like immunoreactivity are present in small and large neurons. The abundance of sodium channel mRNAs in rat DRG is rBI > PN1 >/= PN3 >>> rBIII by quantitative reverse transcription-polymerase chain reaction analysis. Data from reverse transcription-polymerase chain reaction and sequence analyses of human DRG and other human tissues suggest that rat PN1 is an ortholog of the human neuroendocrine channel. In Xenopus oocytes, PN1 exhibits kinetics that are similar to rBIIa sodium currents and is inhibited by tetrodotoxin with an IC50 of 4.3 +/- 0.92 nM. Unlike rBIIa, the inactivation kinetics of PN1 are not accelerated by the coexpression of the beta-subunits. |
| Databáze: | OpenAIRE |
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