Functional characterization of chitin deacetylase 1 gene disrupting larval-pupal transition in the drugstore beetle using RNA interference
| Autor: | Wen-Jia Yang, Xin Yan, Chun-Xu Chen, Yu Cao, Can Li, Kang-Kang Xu, Yong-Lu Meng |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Biology 01 natural sciences Biochemistry Gene Expression Regulation Enzymologic Amidohydrolases 03 medical and health sciences chemistry.chemical_compound Chitin RNA interference Complementary DNA Animals Molecular Biology Gene fungi RNA Gene Expression Regulation Developmental biology.organism_classification Chitin deacetylase Coleoptera 010602 entomology RNA silencing 030104 developmental biology chemistry Larva Insect Proteins RNA Interference Drugstore beetle |
| Zdroj: | Comparative biochemistry and physiology. Part B, Biochemistrymolecular biology. |
| ISSN: | 1879-1107 |
| Popis: | Chitin deacetylases (CDAs) are chitin degradation enzymes that strictly regulate growth and development in insects. In this study, we identified and characterized a full-length cDNA of the CDA gene ( SpCDA1 ) in the drugstore beetle, Stegobium paniceum . The open reading frame of SpCDA1 (1614 bp) encoded a 537 amino acid protein, which possessed typical domain structures of CDAs. Phylogenetic comparison to other insect CDAs revealed that SpCDA1 belongs to Group Ib CDAs. Quantitative real-time PCR analyses showed that SpCDA1 was highly expressed in late larval stages. Significant increase of SpCDA1 transcript level in the larvae was observed upon the exposure of 20-hydroxyecdysone. Injection of double-stranded RNA (dsRNA) of SpCDA1 into the late larvae significantly reduced SpCDA1 transcript levels, resulted in larval-pupal molting difficulty and produced high larval mortality. After 15 days, the survival rate of S. paniceum in ds SpCDA1 group was significantly reduced by 72% compared to the control. The results demonstrated that SpCDA1 is essential for successful larval-pupal transition in S. paniceum and this gene may be a potential target for pest control. |
| Databáze: | OpenAIRE |
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