Repeat Organization and Epigenetic Regulation of the DH-Cμ Domain of the Immunoglobulin Heavy-Chain Gene Locus
| Autor: | Irina Ivanova, Dipanjan Chowdhury, Tirtha Chakraborty, Ranjan Sen, Amanda Keyes, Ramesh Subrahmanyam, Anant Jani |
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| Rok vydání: | 2007 |
| Předmět: |
Genetics
Recombination Genetic biology Heterochromatin Genes Immunoglobulin Heavy Chain Gene Rearrangement B-Lymphocyte Heavy Chain Cell Biology Genetic recombination Chromatin Epigenesis Genetic Protein Structure Tertiary Histones Mice Histone Tandem Repeat Sequences biology.protein Immunoglobulin heavy chain Animals Epigenetics Repeated sequence Immunoglobulin Heavy Chains Promoter Regions Genetic Gene Molecular Biology Cells Cultured |
| Zdroj: | Molecular Cell. 27(5):842-850 |
| ISSN: | 1097-2765 |
| DOI: | 10.1016/j.molcel.2007.07.010 |
| Popis: | Summary The first steps of murine immunoglobulin heavy-chain (IgH) gene recombination take place within a chromosomal domain that contains diversity (D H ) and joining (J H ) gene segments, but not variable (V H ) gene segments. Here we show that the chromatin state of this domain is markedly heterogeneous. Specifically, only 5′- and 3′-most D H gene segments carry active chromatin modifications, whereas intervening D H s are associated with heterochromatic marks that are maintained by ongoing histone deacetylation. The intervening D H s form part of a tandemly repeated sequence that expresses tissue-specific, antisense oriented transcripts. We propose that the intervening D H genes are actively suppressed by repeat-induced epigenetic silencing, which is reflected in their infrequent representation in DJ H junctions compared to the flanking D H genes. |
| Databáze: | OpenAIRE |
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