Electronic cigarette aerosols alter the expression of cisplatin transporters and increase drug resistance in oral cancer cells
Autor: | Lurdes Queimado, Pawan Acharya, Vengatesh Ganapathy, Daniel Zhao, Toral R. Mehta, Célia Bouharati, Balaji Sadhasivam, Jimmy Manyanga |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
medicine.medical_treatment Science Drug resistance Pharmacology Electronic Nicotine Delivery Systems Transport carrier Tobacco smoke Article law.invention 03 medical and health sciences 0302 clinical medicine law medicine Humans Chemotherapy Head and neck cancer Cell Proliferation Cisplatin Aerosols Multidisciplinary Cell Death business.industry Oral cancer Cancer Membrane Transport Proteins medicine.disease Cancer therapeutic resistance 030104 developmental biology Drug Resistance Neoplasm 030220 oncology & carcinogenesis Cancer cell Smoking cessation Medicine Mouth Neoplasms business Electronic cigarette medicine.drug |
Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | Tobacco smoking is the leading preventable cause of cancer. Moreover, continued smoking during cancer therapy reduces overall survival. Aware of the negative consequences of tobacco smoking and the challenges of smoking cessation, cancer patients are inquiring whether they should switch to electronic cigarettes (e-cigarettes). To obtain evidence-based data to inform this decision, we examined the effects of e-cigarette aerosol exposure on cisplatin resistance in head and neck cancer cells. Our results show that cancer cells exposed to e-cigarette aerosol extracts and treated with cisplatin have a significant decrease in cell death, increase in viability, and increase in clonogenic survival when compared to non-exposed cells. Moreover, exposure to e-cigarette aerosol extracts increased the concentration of cisplatin needed to induce a 50% reduction in cell growth (IC50) in a nicotine-independent manner. Tobacco smoke extracts induced similar increases in cisplatin resistance. Changes in the expression of drug influx and efflux transporters, rather than activation of cell growth-promoting pathways or DNA damage repair, contribute to e-cigarette induced cisplatin resistance. These results suggest that like combustible tobacco, e-cigarette use might increase chemotherapy resistance, and emphasize the urgent need for rigorous evaluation of e-cigarettes health effects to ensure evidence-based public health policies. |
Databáze: | OpenAIRE |
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