The walking dead: Is hydroethidine a suitable viability dye for intra-erythrocytic Plasmodium falciparum?
| Autor: | Theresa L. Coetzer, Dewaldt Engelbrecht |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Staining and Labeling
biology Plasmodium falciparum DNA Fragmentation Flow Cytometry Thiazole orange biology.organism_classification Parasite load Molecular biology Parasite Load Phenanthridines Microbiology Infectious Diseases parasitic diseases Animals DNA fragmentation Parasitology Coloring Agents Microscopic morphology Plasmodium species |
| Zdroj: | Parasitology International. 61:731-734 |
| ISSN: | 1383-5769 |
| DOI: | 10.1016/j.parint.2012.07.002 |
| Popis: | Hydroethidine has been used as a viability dye in various haemoparasites, including Plasmodium species. We compared flow cytometric quantification by hydroethidine and thiazole orange in P. falciparum. Dead parasites that did not develop or replicate exhibited high levels of DNA fragmentation and abnormal microscopic morphology, but were detected as viable ring-stage parasites by hydroethidine. Hydroethidine quantification was similar to thiazole orange, a DNA-binding dye that stains live and dead parasites. Data obtained cast concerns on hydroethidine as a suitable viability dye in P. falciparum and highlight the necessity of proper gating in flow cytometric studies quantifying parasitaemia. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect