New Antigens for Serologic Diagnosis of Neuroborreliosis in Children
| Autor: | Pekka Lahdenne, Tuula Lönnqvist, Harri Saxen, Ilkka Seppälä, Heidi Sillanpää, Tero Heikkilä |
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| Rok vydání: | 2005 |
| Předmět: |
Microbiology (medical)
Pathology medicine.medical_specialty Adolescent Lymphocytic pleocytosis Serology 03 medical and health sciences 0302 clinical medicine Cerebrospinal fluid Lyme disease Antigen CSF pleocytosis medicine Humans Lyme Neuroborreliosis 030212 general & internal medicine Child Antigens Bacterial 0303 health sciences biology 030306 microbiology business.industry Infant medicine.disease Antibodies Bacterial Recombinant Proteins 3. Good health Infectious Diseases Immunoglobulin M Borrelia burgdorferi Child Preschool Immunoglobulin G Pediatrics Perinatology and Child Health Immunology biology.protein Antibody business Neuroborreliosis |
| Zdroj: | Pediatric Infectious Disease Journal. 24:709-712 |
| ISSN: | 0891-3668 |
| Popis: | Objective: To evaluate serology with novel Borrelia-specific protein or peptide antigens in the laboratory diagnosis of neuroborreliosis (NB) in children. Methods: The performance of enzyme-linked immunosorbent assays with several recombinant borrelial protein antigens and invariable region 6 synthetic peptide antigen and of a commercial enzyme-linked immunosorbent assay with the flagella antigen were evaluated in the serodiagnosis and follow-up of children with clinical suspicion of NB. Serum samples were obtained from 20 children with neurologic symptoms indicative of NB. The patients were retrospectively divided into 2 groups based on the laboratory tests at presentation indicating definite (n = 7) or probable (n = 13) NB. Results: In addition to cerebrospinal fluid (CSF) lymphocytic pleocytosis and CSF antiflagella antibodies, all 7 patients with definite NB had serum IgG antibodies to at least 2 of the 3 novel antigens at presentation. The 13 patients with probable NB had variable laboratory findings: CSF pleocytosis (n = 7), CSF antiflagella IgM antibodies (n = 4), serum antiflagella IgM and/or IgG antibodies (n = 10). Of these 13 patients, 7 had serum IgG antibodies to 2 of the 3 novel antigens at presentation. During long term follow-up, serum anti-invariable region 6 antibodies disappeared. Conclusions: The present study suggests that assessment of serum antibodies to a panel of Borrelia-specific antigens could improve the laboratory diagnosis of NB at presentation. |
| Databáze: | OpenAIRE |
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