The association of bacterial C9-based TTX-like compounds with Prorocentrum minimum opens new uncertainties about shellfish seafood safety
| Autor: | Margassery Lekha Menon, Luis M. Botana, Alan D. W. Dobson, Juan A. Rubiolo, Stephen A. Jackson, Aristidis Vlamis, Amparo Alfonso, Eva Alonso, María Roel, Inés Rodríguez, Panagiota Katikou |
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| Přispěvatelé: | Universidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Food Safety Food Contamination Tetrodotoxin Article Mass Spectrometry 03 medical and health sciences chemistry.chemical_compound Animals Food science Shellfish Multidisciplinary biology Strain (chemistry) Mass spectrometry Ecology Pseudomonas Dinoflagellate Roseobacter biology.organism_classification Vibrio Bivalvia 030104 developmental biology chemistry Seafood Dinoflagellida Patch clamp Hazard Analysis and Critical Control Points PCR-based techniques Bacteria |
| Zdroj: | Scientific Reports Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela instname |
| Popis: | In 2012, Tetrodotoxin (TTX) was identified in mussels and linked to the presence of Prorocentrum minimum (P. minimum) in Greece. The connexion between TTX and P. minimum was further studied in this paper. First, the presence of TTX-producer bacteria, Vibrio and Pseudomonas spp, was confirmed in Greek mussels. In addition these samples showed high activity as inhibitors of sodium currents (INa). P. minimum was before associated with neurotoxic symptoms, however, the nature and structure of toxins produced by this dinoflagellate remains unknown. Three P. minimum strains, ccmp1529, ccmp2811 and ccmp2956, growing in different conditions of temperature, salinity and light were used to study the production of toxic compounds. Electrophysiological assays showed no effect of ccmp2811 strain on INa, while ccmp1529 and ccmp2956 strains were able to significantly reduce INa in the same way as TTX. In these samples two new compounds, m/z 265 and m/z 308, were identified and characterized by liquid chromatography tandem high-resolution mass spectrometry. Besides, two TTX-related bacteria, Roseobacter and Vibrio sp, were observed. These results show for the first time that P. minimum produce TTX-like compounds with a similar ion pattern and C9-base to TTX analogues and with the same effect on INa Inés Rodríguez is supported by a fellowship from Subprograma de Formación de Personal Investigador MINECO (AGL2012-40185-CO2-01), Spain. The research leading to these results has received funding from the following FEDER cofunded-grants. From CDTI and Technological Funds, supported by Ministerio de Economía y Competitividad, AGL2012-40185-CO2-01, AGL2014-58210-R, and Consellería de Cultura, Educación e Ordenación Universitaria, GRC2013-016. From CDTI under ISIP Programme, Spain, IDI-20130304 APTAFOOD. From the European Union’s Seventh Framework Programme managed by REA – Research Executive Agency (FP7/2007-2013) under grant agreement 312184 PHARMASEA. SI |
| Databáze: | OpenAIRE |
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