Multiple Pathogens Including Potential New Species in Tick Vectors in Côte d’Ivoire
| Autor: | Didier Raoult, Oleg Mediannikov, Jean David N’Guessan, Florence Fenollar, Yaba Louise Achi, Adèle Kacou N’Douba, Mustapha Dahmani, Kouassi Patrick Yao, Nadia Amanzougaghene, Cyrille Bilé Ehounoud |
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| Přispěvatelé: | Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR48, INSB-INSB-Centre National de la Recherche Scientifique (CNRS), Campus International UCAD-IRD [Dakar, Senegal], Université Félix Houphouët-Boigny (UFHB), Ecole de spécialisation en Elevage de Bingerville [Côte D’Ivoire], This study was funded by the IHU Méditerranée Infection (http://en.mediterraneeinfection.com/), UEMOA (PASRES Project) (http://www.csrs.ch/pasres/) and the association APRI (Marseille, France)., Institut des sciences biologiques (INSB-CNRS)-Institut des sciences biologiques (INSB-CNRS)-Centre National de la Recherche Scientifique (CNRS), COMBE, Isabelle |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine lcsh:Arctic medicine. Tropical medicine Anaplasma lcsh:RC955-962 030106 microbiology 030231 tropical medicine Ehrlichia Tick Ehrlichia ruminantium Microbiology 03 medical and health sciences 0302 clinical medicine Ticks [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases Animals Lyme disease Rickettsia Borrelia infection Phylogeny Rickettsia massiliae biology lcsh:Public aspects of medicine Borrelia Public Health Environmental and Occupational Health Correction lcsh:RA1-1270 biology.organism_classification Virology Anaplasmataceae 3. Good health Rhipicephalus Cote d'Ivoire Infectious Diseases Ribosomal RNA [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases Arachnid Vectors Female Hyalomma Amblyomma variegatum Research Article |
| Zdroj: | PLoS Neglected Tropical Diseases PLoS Neglected Tropical Diseases, Public Library of Science, 2016, 10 (1), pp.e0004367. ⟨10.1371/journal.pntd.0004367⟩ PLoS Neglected Tropical Diseases, Vol 10, Iss 1, p e0004367 (2016) PLoS Neglected Tropical Diseases, 2016, 10 (1), pp.e0004367. ⟨10.1371/journal.pntd.0004367⟩ |
| ISSN: | 1935-2727 1935-2735 |
| Popis: | Background Our study aimed to assess the presence of different pathogens in ticks collected in two regions in Côte d’Ivoire. Methodology/Principal Findings Real-time PCR and standard PCR assays coupled to sequencing were used. Three hundred and seventy eight (378) ticks (170 Amblyomma variegatum, 161 Rhipicepalus microplus, 3 Rhipicephalus senegalensis, 27 Hyalomma truncatum, 16 Hyalomma marginatum rufipes, and 1 Hyalomma impressum) were identified and analyzed. We identified as pathogenic bacteria, Rickettsia africae in Am. variegatum (90%), Rh. microplus (10%) and Hyalomma spp. (9%), Rickettsia aeschlimannii in Hyalomma spp. (23%), Rickettsia massiliae in Rh. senegalensis (33%) as well as Coxiella burnetii in 0.2%, Borrelia sp. in 0.2%, Anaplasma centrale in 0.2%, Anaplasma marginale in 0.5%, and Ehrlichia ruminantium in 0.5% of all ticks. Potential new species of Borrelia, Anaplasma, and Wolbachia were detected. Candidatus Borrelia africana and Candidatus Borrelia ivorensis (detected in three ticks) are phylogenetically distant from both the relapsing fever group and Lyme disease group borreliae; both were detected in Am. variegatum. Four new genotypes of bacteria from the Anaplasmataceae family were identified, namely Candidatus Anaplasma ivorensis (detected in three ticks), Candidatus Ehrlichia urmitei (in nine ticks), Candidatus Ehrlichia rustica (in four ticks), and Candidatus Wolbachia ivorensis (in one tick). Conclusions/Significance For the first time, we demonstrate the presence of different pathogens such as R. aeschlimannii, C. burnetii, Borrelia sp., A. centrale, A. marginale, and E. ruminantium in ticks in Côte d’Ivoire as well as potential new species of unknown pathogenicity. Author Summary The management of febrile illnesses represents a veritable challenge in sub Saharan-Africa. Until recently most of them were considered as malaria. However, it was showed that a large part of non-malarial febrile diseases in African rural regions (for instance, in Senegal) may be caused by tick-borne infections. Unfortunately, no data exist about the prevalence and incidence of tick-borne diseases in Côte d'Ivoire and their role in public health. We aimed to search for different pathogenic bacteria in ticks in order to understand if there is the background for tick-borne diseases. We detected pathogenic bacteria responsible for many infectious diseases like Rickettsia (spotted fevers), Borrelia (relapsing fevers), Anaplasma, Ehrlichia (ehrlichiosis and anaplasmosis) and Coxiella burnetii (Q fever). These finding suggested that, as in others sub-Saharan African countries, tick-borne disease may be considered as a health care problem in Cote d'Ivoire. |
| Databáze: | OpenAIRE |
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