Efficient decolorization of recalcitrant dyes at neutral/alkaline pH by a new bacterial laccase-mediator system

Autor: Fernando Battaglini, Lucy L. Coria-Oriundo, Sonia Alejandra Wirth
Rok vydání: 2021
Předmět:
Bacillus subtilis CotA
Health
Toxicology and Mutagenesis
0211 other engineering and technologies
Phenothiazine
Streptomyces ipomoeae SilA
Anthraquinones
02 engineering and technology
010501 environmental sciences
Indigo Carmine
01 natural sciences
Remazol Brilliant Blue R
Environmental pollution
chemistry.chemical_compound
Tap water
Escherichia coli
GE1-350
Malachite green
Coloring Agents
0105 earth and related environmental sciences
Laccase
021110 strategic
defence & security studies
Temperature
Public Health
Environmental and Occupational Health
Xylidine
General Medicine
Hydrogen-Ion Concentration
Pollution
Combinatorial chemistry
Environmentally friendly
Streptomyces
Environmental sciences
Biodegradation
Environmental
Dye decolorization
TD172-193.5
chemistry
Indigo carmine
Degradation (geology)
Azo Compounds
Oxidation-Reduction
Zdroj: Ecotoxicology and Environmental Safety, Vol 217, Iss, Pp 112237-(2021)
ISSN: 0147-6513
DOI: 10.1016/j.ecoenv.2021.112237
Popis: Laccases and laccase-mediator systems (LMS) are versatile catalysts that can oxidize a broad range of substrates coupled to the sole reduction of dioxygen to water. They possess many biotechnological applications in paper, textile, and food industries, bioethanol production, organic synthesis, detection and degradation of pollutants, and biofuel cell development. In particular, bacterial laccases are getting relevance due to their activity in a wide range of pH and temperature and their robustness under harsh conditions. However, the enzyme and the redox mediator's availability and costs limit their large-scale commercial use. Here we demonstrate that β-(10-phenothiazyl)-propionic acid can be used as an efficient and low-cost redox mediator for decolorizing synthetic dyes by the recombinant laccase SilA from Streptomyces ipomoeae produced in E. coli. This new LMS can decolorize more than 80% indigo carmine and malachite green in 1 h at pH = 8.0 and 2 h in tap water (pH = 6.8). Furthermore, it decolorized more than 40% of anthraquinone dye remazol brilliant blue R and 80% of azo dye xylidine ponceau in 5 h at 50 °C, pH 8.0. It supported at least 3 decolorization cycles without losing activity, representing an attractive candidate for a cost-effective and environmentally friendly LMS functional at neutral to alkaline pH.
Databáze: OpenAIRE
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