Microbiological Investigation in Teeth with Persistent/Secondary Endodontic Infection in Different Stages of Root Canal Retreatment
| Autor: | Marlos Barbosa-Ribeiro, Lidiane Mendes Louzada, José Flávio Affonso de Almeida, Brenda Paula Figueiredo de Almeida Gomes, Adriana de-Jesus-Soares, Caio Cezar Randi Ferraz, Marina Angélica Marciano, Rodrigo Arruda-Vasconcelos, Alexandre Augusto Zaia, Augusto Rodrigues Lima |
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| Rok vydání: | 2020 |
| Předmět: |
Endodontic therapy
Dental Assisting Root canal persistent endodontic infection Dentistry Bacillus Orthodontics endodontic treatment Calcium Hydroxide Forsythia Medicine Humans Dentistry (miscellaneous) General Dentistry Periodontitis Colony-forming unit biology Bacteria business.industry intracanal medication medicine.disease biology.organism_classification Root Canal Therapy stomatognathic diseases nested PCR medicine.anatomical_structure Retreatment Dental Hygiene Periodontics Original Article Oral Surgery Dental Pulp Cavity business Nested polymerase chain reaction Periapical Periodontitis |
| Zdroj: | European Endodontic Journal |
| ISSN: | 2548-0839 |
| Popis: | Objective: The present clinical study investigated the microbiota of teeth with persistent secondary endodontic infection in the different phases of root canal retreatment. Methods: Twenty filled single-rooted teeth with apical periodontitis were included. Samples were collected with sterile paper points before chemo-mechanical preparation (CMP) (S1), after CMP (S2) and after 30 days of calcium hydroxide-based intracanal medication (ICM) (S3). Cultivable bacteria were assessed by colony forming units count (CFU/mL). DNA was extracted and assessed by using nested PCR. Paired t-test and repeated measures ANOVA were applied for intragroup analysis in the stages of endodontic therapy at a significance level of 5%. Results: Cultivable bacteria were detected in all initial samples. CMP reduced bacteria by 99.4% and ICM by 99.5%. The most prevalent species found in the initial samples were E. faecalis (20/20), P. gingivalis (20/20), F. nucleatum (17/20) and A. actinomycetemcomitans (10/20), whereas D. pneumosintes, F. alocis, P. nigrescens and T. socranskii were not detected. After CMP, A. israelii, A. naeslundii, G. morbillorum, T. forsythia and T. denticola were not detected (P0.05) and F. nucleatum had its DNA significantly reduced after CMP (P |
| Databáze: | OpenAIRE |
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