Detection of group B streptococcus: Comparison of an optical immunoassay with direct plating and broth-enhanced culture methods
| Autor: | Thomas D. Myles, Tuan M. Nguyen, Bahij Nuwayhid, Daniel W. Gauthier, Marlos A. G. Viana, Paul C. Schreckenberger |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
medicine.diagnostic_test
Streptococcus business.industry Obstetrics and Gynecology Gold standard (test) medicine.disease_cause Predictive value Group B Microbiology chemistry.chemical_compound chemistry Immunoassay Direct plating Pediatrics Perinatology and Child Health medicine Trypticase soy agar business Sheep blood |
| Zdroj: | Journal of Maternal-Fetal and Neonatal Medicine. 7:172-176 |
| ISSN: | 1476-4954 1476-7058 |
| DOI: | 10.3109/14767059809020436 |
| Popis: | The aim of this study was to compare the diagnostic accuracy of an optical immunoassay (STREP B OIA, Biostar) to direct plating and broth-enhanced culture for the detection of group B streptococcus (CBS) colonization of the lower genital tract in pregnant women. GBS cultures from the lower genital tract were obtained in a prospective fashion using a dual swab transport system from patients with risk factors for perinatal GBS infection. One swab was used to inoculate a trypticase soy agar plate with 5% sheep blood (TSA) and then placed in Lim broth. The other swab was used to perform the Strep B OIA. Growth of GBS by either direct plating or broth-enhanced culture was used as the gold standard for determining GBS colonization. Of the 524 women in the study, 90 women had positive cultures (either TSA or Lim broth). The sensitivity, specificity, positive predictive value, and negative predictive value of the Strep B OIA were 47% (42/90), 96% (416/434), 70% (42/60), 90% (416/464). The sensitivity, specificity... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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