Stimulation ofiptoverexpression as a tool to elucidate the role of cytokinins in high temperature responses ofArabidopsis thaliana
| Autor: | Jana Dobrá, Eva Ge, Jan Novák, Petre I. Dobrev, Martin Černý, Radomira Vankova, Jan Skalák, Marie Hronková, Břetislav Brzobohatý, Petr Jedelský |
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| Rok vydání: | 2016 |
| Předmět: |
Proteomics
0106 biological sciences 0301 basic medicine Cytokinins Hot Temperature Arabidopsis thaliana Physiology Arabidopsis Plant Science Plant Roots 01 natural sciences Dexamethasone proteome heat stress Transcriptome Abscisic acid cytokinin 03 medical and health sciences chemistry.chemical_compound Plant Growth Regulators Gene Expression Regulation Plant Gene expression heterocyclic compounds Heat shock Alkyl and Aryl Transferases biology Gene Expression Profiling fungi food and beverages biology.organism_classification Molecular biology 030104 developmental biology chemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Proteome Cytokinin isopentenyltransferase Heat-Shock Response Plant Shoots Research Paper Signal Transduction 010606 plant biology & botany |
| Zdroj: | Journal of Experimental Botany |
| ISSN: | 1460-2431 0022-0957 |
| Popis: | Highlight Decisive role for cytokinins in stimulation of transpiration during the early heat stress response was demonstrated. Longer term positive effects of cytokinins were associated with elevation of stress- and photosynthesis-related proteins and transcripts. Cytokinins (CKs) are phytohormones regulating plant growth and development as well as response to the environment. In order to evaluate their function in heat stress (HS) responses, the effect of CK elevation was determined during three types of HS – targeted to shoots, targeted to roots and applied to the whole plant. The early (30min) and longer term (3h) responses were followed at the hormonal, transcriptomic and proteomic levels in Arabidopsis transformants with dexamethasone-inducible expression of the CK biosynthetic gene isopentenyltransferase (ipt) and the corresponding wild-type (Col-0). Combination of hormonal and phenotypic analyses showed transient up-regulation of the CK/abscisic acid ratio, which controls stomatal aperture, to be more pronounced in the transformant. HS responses of the root proteome and Rubisco-immunodepleted leaf proteome were followed using 2-D gel electrophoresis and MALDI-TOF/TOF. More than 100 HS-responsive proteins were detected, most of them being modulated by CK increase. Proteome and transcriptome analyses demonstrated that CKs have longer term positive effects on the stress-related proteins and transcripts, as well as on the photosynthesis-related ones. Transient accumulation of CKs and stimulation of their signal transduction in tissue(s) not exposed to HS indicate that they are involved in plant stress responses. |
| Databáze: | OpenAIRE |
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