The Isolation of Vacuoles from Candida Utilis
| Autor: | Nakamura Kd |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Cytoplasm
S-Adenosylmethionine Neutral red Time Factors Spheroplasts Vacuole Glucosephosphate Dehydrogenase Biology Cell Fractionation Micrography Biochemistry Microbiology chemistry.chemical_compound Methods Genetics Candida Differential centrifugation Microscopy Ultraviolet Spheroplast Isolation (microbiology) Yeast Mitochondria Organoids Succinate Dehydrogenase Alcohol Oxidoreductases Cytolysis chemistry Spectrophotometry Spectrophotometry Ultraviolet |
| Zdroj: | Preparative Biochemistry. 3:553-561 |
| ISSN: | 0032-7484 |
| DOI: | 10.1080/00327487308061537 |
| Popis: | Vacuoles containing S-adenosylmethionine have been isolated from the yeast, Candida utilis, by the osmotic lysis of its spheroplasts and differential centrifugation. Culture in the presence of L-methionine and harvest in the stationary phase contributed to the ease of isolation and stability of the vacuoles. The vacuoles showed retentiveness for the sulfonium compound and responded to the vital-staining reaction with Neutral Red. Ultraviolet micrography indicated the homogeneity of the vacuolar preparation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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