Chiral determination of 3,4-methylenedioxypyrovalerone enantiomers in rat serum
| Autor: | S. Michael Owens, Michael D. Hambuchen, Howard P. Hendrickson |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Chromatography
Chemistry Calibration curve General Chemical Engineering 010401 analytical chemistry General Engineering Tandem mass spectrometry Mass spectrometry 01 natural sciences Article 0104 chemical sciences Analytical Chemistry Chiral column chromatography 03 medical and health sciences 0302 clinical medicine Pharmacokinetics Racemic mixture Enantiomer Racemization 030217 neurology & neurosurgery |
| Zdroj: | Analytical Methods. 9:609-617 |
| ISSN: | 1759-9679 1759-9660 |
| DOI: | 10.1039/c6ay03176e |
| Popis: | The emerging stimulant drug of abuse (3,4)-methylenedioxypyrovalerone [(R,S)-MDPV] is self-administered as a racemic mixture by intranasal, iv, oral, and smoking routes. The individual enantiomers are known to have widely different pharmacological effects, with (S)-MDPV showing much greater potency than (R)-MDPV in pharmacological testing. The goal of these studies was to develop and validate an analytical method for quantitation of (R)-MDPV, (S)-MDPV and (R,S)-MDPV in small volumes of rat serum using a chiral separation column and liquid chromatography-mass spectrometry. The method was validated for selectivity, precision, accuracy, recovery, sensitivity, and reproducibility. The method was also used to determine the enantiomeric stability of the individual enantiomers during sample cleanup and analysis. The linear dynamic range of the calibration curve was 1 – 1000 ng/ml for each enantiomer. Concentration values for the lower limit of quantitation (1 ng/ml) were within 30% of their nominal value, but all other calibration standards were |
| Databáze: | OpenAIRE |
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