Opsonin-Deficient Nucleoproteic Corona Endows UnPEGylated Liposomes with Stealth Properties In Vivo
| Autor: | Francesca Giulimondi, Elisabetta Vulpis, Luca Digiacomo, Maria Valeria Giuli, Angelica Mancusi, Anna Laura Capriotti, Aldo Laganà, Andrea Cerrato, Riccardo Zenezini Chiozzi, Carmine Nicoletti, Heinz Amenitsch, Francesco Cardarelli, Laura Masuelli, Roberto Bei, Isabella Screpanti, Daniela Pozzi, Alessandra Zingoni, Saula Checquolo, Giulio Caracciolo |
|---|---|
| Přispěvatelé: | Giulimondi, Francesca, Vulpis, Elisabetta, Digiacomo, Luca, Giuli, Maria Valeria, Mancusi, Angelica, Capriotti, Anna Laura, Laganà, Aldo, Cerrato, Andrea, Zenezini Chiozzi, Riccardo, Nicoletti, Carmine, Amenitsch, Heinz, Cardarelli, Francesco, Masuelli, Laura, Bei, Roberto, Screpanti, Isabella, Pozzi, Daniela, Zingoni, Alessandra, Checquolo, Saula, Caracciolo, Giulio |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
liposomes
lipoplexes General Engineering immune cell interactions General Physics and Astronomy stealth nanoparticles Opsonin Proteins Settore MED/04 Settore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin) Polyethylene Glycols protein corona gene delivery system liposome Humans General Materials Science gene delivery systems immune cell interaction lipoplexe |
| Popis: | For several decades, surface grafted polyethylene glycol (PEG) has been a go-to strategy for preserving the synthetic identity of liposomes in physiological milieu and preventing clearance by immune cells. However, the limited clinical translation of PEGylated liposomes is mainly due to the protein corona formation and the subsequent modification of liposomes’ synthetic identity, which affects their interactions with immune cells and blood residency. Here we exploit the electric charge of DNA to generate unPEGylated liposome/DNA complexes that, upon exposure to human plasma, gets covered with an opsonin-deficient protein corona. The final product of the synthetic process is a biomimetic nanoparticle type covered by a proteonucleotidic corona, or “proteoDNAsome”, which maintains its synthetic identity in vivo and is able to slip past the immune system more efficiently than PEGylated liposomes. Accumulation of proteoDNAsomes in the spleen and the liver was lower than that of PEGylated systems. Our work highlights the importance of generating stable biomolecular coronas in the development of stealth unPEGylated particles, thus providing a connection between the biological behavior of particles in vivo and their synthetic identity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|