In vitro reconstruction of tetronate RK-682 biosynthesis
| Autor: | Yuliya Demydchuk, James Chettle, Manuela Tosin, Hiroyuki Osada, Peter F. Leadlay, Frank Hahn, Yuhui Sun |
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| Rok vydání: | 2009 |
| Předmět: |
010402 general chemistry
Ring (chemistry) 01 natural sciences Article Substrate Specificity Polyketide chemistry.chemical_compound Bacterial Proteins Biosynthesis Phosphoprotein Phosphatases Enzyme Inhibitors Molecular Biology chemistry.chemical_classification Molecular Structure biology 010405 organic chemistry In vitro toxicology Cell Biology biology.organism_classification In vitro 0104 chemical sciences Saccharopolyspora Enzyme chemistry Biochemistry Multigene Family Assembly line |
| Zdroj: | Nature chemical biology |
| ISSN: | 1552-4469 1552-4450 |
| DOI: | 10.1038/nchembio.285 |
| Popis: | The protein phosphatase inhibitor RK-682 is one of a number of potentially valuable tetronate polyketide natural products. Understanding how the tetronate ring is formed has been frustrated by the inaccessibility of the putative substrates. We report the heterologous expression of rk genes in Saccharopolyspora erythraea and reconstitution of the RK-682 pathway using recombinant enzymes, and we show that RkD is the enzyme required for RK-682 formation from acyl carrier protein-bound substrates. |
| Databáze: | OpenAIRE |
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