Extracellular Vesicles Derived from Adipose-Derived Stem Cells Accelerate Diabetic Wound Healing by Suppressing the Expression of Matrix Metalloproteinase-9
| Autor: | Shu Wu, Yangyan Yi, Yuanzheng Zhu, Xuan Hu, Jiaying Nie, Zhaohui Wang, Jiangwen Wang |
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| Rok vydání: | 2022 |
| Předmět: |
Wound Healing
medicine.diagnostic_test Chemistry Stem Cells Pharmaceutical Science Adipose tissue Matrix (biology) Pharmacology Extracellular Vesicles Mice HaCaT Adipose Tissue Matrix Metalloproteinase 9 Western blot Trichrome In vivo Diabetes Mellitus medicine Animals Stem cell Wound healing Biotechnology |
| Zdroj: | Current Pharmaceutical Biotechnology. 23:894-901 |
| ISSN: | 1389-2010 |
| DOI: | 10.2174/1389201022666210719154009 |
| Popis: | Background: The healing of diabetic wounds is poor due to a collagen deposition disorder. Matrix metalloproteinase-9 (MMP-9) is closely related to collagen deposition in the process of tissue repair. Many studies have demonstrated that extracellular vesicles derived from adipose-derived stem cells (ADSC-EVs) promote diabetic wound healing by enhancing collagen deposition. Objective: In this study, we explored whether ADSC-EVs could downregulate the expression of MMP-9 in diabetic wounds and promote wound healing by improving collagen deposition. The potential effects of ADSC-EVs on MMP-9 and diabetic wound healing were tested both in vitro and in vivo. Methods: We first evaluated the effect of ADSC-EVs on the proliferation and MMP-9 secretion of HaCaT cells treated with advanced glycation end product-bovine serum albumin (AGE-BSA) using CCK-8, western blot and MMP-9 enzyme-linked immunosorbent assay(ELISA). Next, the effects of ADSC-EVs on healing, re-epithelialisation, collagen deposition, and MMP-9 concentration in diabetic wound fluids were evaluated in an immunodeficient mouse model via MMP-9 ELISA and haematoxylin and eosin, Masson’s trichrome, and immunofluorescence staining for MMP-9. Results: In vitro, ADSC-EVs promoted the proliferation and MMP-9 secretion of HaCaT cells. In vivo, ADSC-EVs accelerated diabetic wound healing by improving re-epithelialisation and collagen deposition and by inhibiting the expression of MMP-9. Conclusion: ADSC-EVs possess the potential of healing of diabetic wounds in a mouse model by inhibiting downregulating MMP-9 and improving collagen deposition. Thus, ADSC-EVs are a promising candidate for the treatment of diabetic wounds. |
| Databáze: | OpenAIRE |
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