Regulation of the secretion of immunoregulatory factors of mesenchymal stem cells (MSCs) by collagen-based scaffolds during chondrogenesis
| Autor: | Xuening Chen, Jingyu Yang, Tun Yuan, Xingdong Zhang, Xiao Yang, Yujiang Fan |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Materials science Cell Survival Cellular differentiation Connective tissue Enzyme-Linked Immunosorbent Assay Bioengineering 02 engineering and technology Rats Sprague-Dawley Biomaterials 03 medical and health sciences Tissue engineering medicine Animals Immunologic Factors Cell Lineage Secretion Cell Proliferation chemistry.chemical_classification Tissue Scaffolds Gene Expression Profiling Scleroprotein Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells 021001 nanoscience & nanotechnology Chondrogenesis Cell biology 030104 developmental biology medicine.anatomical_structure Solubility chemistry Mechanics of Materials Immunology Cattle Collagen Stem cell 0210 nano-technology |
| Zdroj: | Materials Science and Engineering: C. 70:983-991 |
| ISSN: | 0928-4931 |
| DOI: | 10.1016/j.msec.2016.04.096 |
| Popis: | In the latest decade, mesenchymal stem cells (MSCs) have wildly considered as a source of seeded cells in tissue engineering, not only because of its multi-differentiation potentials, but also due to its immunoregulation ability. The main immunoregulatory features of MSCs could be divided into low self-immunogenicity and secretion of soluble factors. In this study, we explored how scaffold structures modulated the secretion of soluble immunoregulatory factors in MSCs under an allogeneic cartilage tissue engineering background. MSCs were seeded in four different collagen-based scaffolds. Their proliferation, differentiation, and secretion of various soluble factors associated with the immunosuppressive effects were evaluated. In this study, qRT-PCR, ELISA and immunoregulation results showed a great variability of the factor secretion by MSCs seeded in scaffolds with different structures. Compared with two-dimensional (2D) monolayer culture condition, three-dimensional (3D) groups (hydrogels and sponge) could effectively promote the mRNA expression and the protein production of soluble immune-related factors. Also, the supernatants collected from 3D groups obviously showed inhibition on allogeneic lymphocyte activating. These results suggested that scaffold structures might modulate MSCs' secretion of soluble immunoregulatory factors, and our study might enlighten the scaffold designs for desired tissue regeneration to control the host immune rejection through immune-regulation reaction. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect