Chemical sympathectomy by 6-OH dopamine during fetal life results in inguinal testis through altering cremasteric contractility in rats
| Autor: | Saniye Ekinci, Selçuk Otçu, F.Cahit Tanyel, Rustu Onur, Mert Ertunc, Murat Yildirim |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Male
medicine.medical_specialty Sympathetic nervous system Offspring Nitroarginine Contractility Pregnancy Internal medicine Isoprenaline Cryptorchidism Testis Scrotum medicine Animals Enzyme Inhibitors Sympathomimetics Oxidopamine business.industry Isoproterenol Sympathectomy Chemical Muscle Smooth General Medicine Calcium Channel Blockers Rats medicine.anatomical_structure Endocrinology Verapamil Prenatal Exposure Delayed Effects Pediatrics Perinatology and Child Health Cremaster muscle Sympatholytics Catecholamine Female Surgery business Muscle Contraction medicine.drug |
| Zdroj: | Journal of Pediatric Surgery. 38:1628-1632 |
| ISSN: | 0022-3468 |
| DOI: | 10.1016/s0022-3468(03)00574-8 |
| Popis: | Androgens are proposed to influence testicular descent through modulating sympathetic tone. An experimental study was undertaken to evaluate the effects of prenatal chemical sympathectomy on testicular location associated with the alterations in contractile properties of cremaster muscles in rats.Time-mated pregnancies were started in 10 rats. Two groups, each receiving saline or 6-hydroxydopamine from day 15 to day 19 of intrauterine life were established. At 2 months of age, localization of testes were evaluated, cremaster muscles were removed, and contractile properties were studied. Twitch and tetanic contractions were recorded isometrically at 37 degrees C. Effects of verapamil, isoprenaline, and L-NNA were investigated. Results were compared through analysis of variance (ANOVA), and P values less than.05 were considered to be significant.Both testes of all male offspring in the control group (n = 19) were in the scrotum. Six offspring among 17 subjected to 6-hydroxydopamine had undescended testes. Treatment with 6-hydroxydopamine had no effect on force-frequency relationship of cremaster muscle strips. Cremaster muscles of rats exposed to 6-hydroxydopamine had lower sensitivity to voltage-sensitive Ca++ channel blockade by verapamil (3 x 10(4) mol/L; P.05). These muscles displayed greater contractile response to isoprenaline (10(-5) mol/L; P.05) but not to nitric oxide synthase inhibition by N(omega)-nitro-L-arginine. Alterations in contractile properties of the muscles did not differ according to localization of testes among rats subjected to 6-hydroxydopamine.Administration of 6-hydroxydopamine resulted in suprascrotally located testes. This localization has been associated with less exposure at sympathetic tonus. These findings support that sympathetic activity plays an important role in localization of testis. |
| Databáze: | OpenAIRE |
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