Determination of EBV serostatus prior to kidney transplantation: Comparison of VIDAS®, LIAISON® and immunofluorescence assays
| Autor: | Ingolfur Johannessen, S Black, A Galloway, M Noel, Cat Graham, M Shearman |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Epstein-Barr Virus Infections Herpesvirus 4 Human medicine.medical_specialty Adolescent medicine.medical_treatment Fluorescent Antibody Technique Enzyme-Linked Immunosorbent Assay Biology Antibodies Viral Immunofluorescence Sensitivity and Specificity Gastroenterology Serology Young Adult Virology Internal medicine medicine Humans Serologic Tests Epstein–Barr virus infection Kidney transplantation Aged Aged 80 and over Automation Laboratory medicine.diagnostic_test Immunosuppression Middle Aged medicine.disease Kidney Transplantation Transplantation biology.protein Female Antibody Serostatus |
| Zdroj: | Journal of Virological Methods. 203:107-111 |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/j.jviromet.2014.03.022 |
| Popis: | Immunosuppression following solid organ transplantation reduces T cell-mediated immune control of Epstein–Barr Virus (EBV), which may then drive development of post-transplant lymphoproliferative disease. Serology plays a key role in determination of risk of outgrowth of such lesions following transplantation. The study compared the VIDAS ® (bioMerieux) and LIAISON ® (DiaSorin) enzyme immunoassays (EIAs) and immunofluorescence assays (IFA; MBL-Bion) in the kidney transplantation setting. Sera from 100 live kidney donors [51 males; age range 20–82 years (mean 51.2 years)] and 100 cadaveric kidney recipients [70 males; age range 17–77 years (mean 51.0 years)] were tested. Overall proportional agreement ranged from 96% to 100% for VIDAS ® and LIAISON ® . Sensitivity ranged from 91% to 100% and 92% to 100% for VIDAS ® /IFA and LIAISON ® /IFA, respectively. The VIDAS ® and LIAISON ® approaches gave similar results. Such automated random access EIAs are well suited to busy clinical virology laboratories and rapid determination of donor and recipient EBV serostatus prior to transplantation. |
| Databáze: | OpenAIRE |
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