β-Glucan improves wound healing in silver catfish (Rhamdia quelen)
| Autor: | Rafael Frandoloso, Cláudia Cerutti Dazzi, Ana Paula dos Santos Voloski, Fernando Jonas Sutili, Lucas de Figueiredo Soveral, Luiz Carlos Kreutz |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty beta-Glucans Inflammation Aquatic Science Biology Andrology 03 medical and health sciences Dermis Immersion medicine Environmental Chemistry Animals Fibroblast Catfishes Wound Healing integumentary system Granulation tissue Aquatic animal 04 agricultural and veterinary sciences General Medicine 030104 developmental biology medicine.anatomical_structure 040102 fisheries 0401 agriculture forestry and fisheries Histopathology Female medicine.symptom Wound healing Catfish |
| Zdroj: | Fishshellfish immunology. 93 |
| ISSN: | 1095-9947 |
| Popis: | The immune modulating activity of β-glucan on aquatic species has been a matter of intense investigation. Here, we aimed to investigate the effect of β-glucan on wound healing of silver catfish, a Neotropical South American scale-free fish. Small sections of skin and muscle (3 mm in diameter) were removed and fish were bathed daily with β-glucan (0.1% and 0.5%) up to 28 days when cicatrization was complete. A group of fish similarly injured and non-exposed to β-glucan was used as control. Wound closure and healing was monitored visually and by histopathological analysis. In fish bathed with 0.5% β-glucan we found reduced blood cortisol levels at day one post-wounding and, by day 7 post wounding, the deposition of granulation tissue was higher compared to non-exposed fish. In addition, from day 7 forward, wound size was significantly lower in fish bathed with 0.5% β-glucan. Histopathological analysis of the wounded site indicated a thin layer of immature epidermal cells at day one post wounding. A discrete inflammation with mixed inflammatory cell infiltrate was observed on wounded muscle and was lower by day 7 post wounding on fish bathed with 0.5% β-glucan. By day 14 post wounding, the deposition of collagen fibers and the presence of fibroblast and new muscle fibers were higher in fish exposed to 0.5% β-glucan, and dermis restoration was complete. Thus, our results indicate that in silver catfish wound healing occurs rapidly and improves greatly by daily bathing with β-glucan. |
| Databáze: | OpenAIRE |
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