Malaysian endophytic fungal extracts-induced anti-inflammation in Lipopolysaccharide-activated BV-2 microglia is associated with attenuation of NO production and, IL-6 and TNF-α expression
| Autor: | Sharmili Vidyadaran, Siong Meng Lim, Kalavathy Ramasamy, Azzeme Harun, Anthony L. J. Cole |
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| Rok vydání: | 2015 |
| Předmět: |
Lipopolysaccharides
Lipopolysaccharide Cell Survival medicine.medical_treatment Anti-Inflammatory Agents Nitric Oxide Synthase Type II Inflammation Biology Cell Line Proinflammatory cytokine Microbiology chemistry.chemical_compound Endophytes CD40 medicine Viability assay CD40 Antigens Neuroinflammation Cinnamomum Biological Products Microglia Interleukin-6 Tumor Necrosis Factor-alpha Macrophages Fungi NF-kappa B Nitric oxide General Medicine Endophyte Neuroprotective Agents Cytokine medicine.anatomical_structure Complementary and alternative medicine chemistry Immunology Cytokines Tumor necrosis factor alpha Inflammation Mediators medicine.symptom Research Article |
| Zdroj: | BMC Complementary and Alternative Medicine |
| ISSN: | 1472-6882 |
| DOI: | 10.1186/s12906-015-0685-5 |
| Popis: | Background Excessive production of inflammatory mediators such as nitric oxide (NO) and proinflammatory cytokines like tumour necrosis factor-alpha (TNF-α) from activated microglia contributes to uncontrolled inflammation in neurodegenerative diseases. This study investigated the protective role of five endophytic extracts (HAB16R12, HAB16R13, HAB16R14, HAB16R18 and HAB8R24) against LPS-induced inflammatory events in vitro. These endophytic extracts were previously found to exhibit potent neuroprotective effect against LPS-challenged microglial cells. Methods The effects of these fungal endophytic extracts against nitric oxide (NO), CD40 phenotype and, pro- and anti-inflammatory cytokine production in lipopolysaccharide (LPS)-stimulated BV2 microglia cells were examined using commercially available assay kits, immunophenotyping and flow cytometry, respectively. Results Microglia pre-treated with the five endophytic extracts (0.1 mg/mL) reduced NO production without compromising cell viability. Whilst CD40 expression in LPS-stimulated microglia was not significantly different with or without the influence of endophytic extracts, expression of the proinflammatory cytokines, IL-6 and TNF-α in LPS-stimulated microglia was significantly (P |
| Databáze: | OpenAIRE |
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