Pretreatment with anti-flagellin serum delays acute lung injury in rats with sepsis
| Autor: | Chang-Xi Zhou, Qi Li, Mingdong Hu, Jiancheng Xu, Guisheng Qian, Mei Mao, Wang Yi, Yu Yang |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Serum medicine.medical_specialty Allergy Immunology Pharmacology toxicology Acute Lung Injury Lung injury Sepsis Internal medicine medicine Animals RNA Messenger Rats Wistar Pharmacology biology business.industry Tumor Necrosis Factor-alpha respiratory system medicine.disease Rheumatology respiratory tract diseases Rats Toll-Like Receptor 5 TLR5 biology.protein business Bronchoalveolar Lavage Fluid Flagellin |
| Zdroj: | Inflammation research : official journal of the European Histamine Research Society ... [et al.]. 61(8) |
| ISSN: | 1420-908X |
| Popis: | This study examined the reduction of sepsis-induced ALI by inhibition of flagellin-stimulated TLR5 signaling.Rats were randomly divided into three groups: one group served as the sham-operated group (control group), and the other two groups received the induction of sepsis (sepsis and treatment groups). The treatment group was injected with anti-flagellin serum before induction of sepsis. At 2, 4, 6, 12, 24, and 48 h following induction of sepsis (six time-point subgroups, n = 10 per subgroup), arterial PaO(2), wet/dry (W/D) lung weight ratios, levels of serum and BALF flagellin and TNF-α, pulmonary pathological alterations, and TLR5 mRNA expression in the lungs were examined.Compared to sham-operated rats, septic rats had: increased levels of serum and BALF flagellin at 6, 12, 24, and 48 h; reduced arterial PaO(2); elevated W/D lung weight ratio; increased serum and BALF TNF-α levels; and up-regulated TLR5 mRNA expression at 12, 24, and 48 h (P0.01). Pretreatment with anti-flagellin serum, however, significantly inhibited sepsis-associated declines in arterial PaO(2), increased W/D lung weight ratios, elevated serum and BALF TNF-α levels, and up-regulated TLR5 mRNA expression at 24 and 48 h (P0.01).Neutralizing the actions of circulating flagellin with anti-flagellin serum delayed the development of ALI in rats with sepsis. |
| Databáze: | OpenAIRE |
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