Sequences and repertoire of human T cell receptor alpha chain variable region genes in mature T lymphocytes
| Autor: | Tak W. Mak, Nobuhiro Kimura, Barry Toyonaga, Yasunobu Yoshikai |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1986 |
| Předmět: |
Sequence analysis
Immunology Immunoglobulin Variable Region Receptors Antigen T-Cell Alpha (ethology) Somatic hypermutation Biology Immunoglobulin alpha-Chains Complementary DNA T-Cell Receptor Alpha Chain Immunology and Allergy Humans Amino Acid Sequence Cloning Molecular Gene Genetics Electrophoresis Agar Gel Base Sequence Collodion Nucleic Acid Hybridization Articles DNA Molecular biology Genes Immunoglobulin heavy chain Immunoglobulin Heavy Chains Alpha chain |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| Popis: | 24 human T cell receptor alpha chain messages have been examined by cDNA sequence analysis and Southern blot. The data indicate that there are approximately 40 alpha chain T cell receptor variable gene segments, which can be divided into 12 families. Comparison of the J gene segments from the cDNAs to previously determined germline J alpha sequences places the number of J alpha gene segments over 21, and indicates their number to be approximately 55. Identical nucleotide sequences in independent isolates of V alpha and J alpha gene segments indicate that hypermutation may not be a common mechanism for the expansion of diversity in these genes, and suggest that the major source of diversity within the alpha chain repertoire is a result of recombinational joinings between germline V alpha and J alpha sequences, combined with imprecise junctional joining. Analysis of the V regions of these alpha chain messages reveals the presence of three domains of hypervariability roughly analogous to the CDR1, CDR2, and CDR3 regions of immunoglobulin. |
| Databáze: | OpenAIRE |
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