| Autor: |
Javed Akhter, Min Shi, Steven H. Myint, S.M. Hussain Qadri |
| Rok vydání: |
1995 |
| Předmět: |
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| Zdroj: |
Clinical and Diagnostic Virology. 3:215-220 |
| ISSN: |
0928-0197 |
| DOI: |
10.1016/s0928-0197(94)00038-7 |
| Popis: |
Background Astroviruses are becoming increasingly recognised as a cause of diarrhoea in children, and to a lesser extent, adults. Electron microscopy is the recognised standard for diagnosis but is not available in many centres. Objectives This study set out to evaluate a RT-PCR method for the diagnosis of astrovirus in faecal specimens. Study design 130 diarrhoeal stools were collected prospectively both from Saudi Arabia and Leicester, UK. The patients from each centre were age-matched. Twenty control isolates from food service staff without clinical illness were also collected. Both electron microscopy (EM) and RT-PCR (for astrovirus serotype 1) were used to diagnose astrovirus infection. Results Of 23 PCR-positive specimens, only 17 were detected by EM. The prevalence of astrovirus serotype 1 was determined by PCR as 1.5% in Saudi Arabia and 4.6% in the UK. Conclusion RT-PCR is a more sensitive alternative to EM for astrovirus serotype 1. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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