Reciprocal cybrids reveal how organellar genomes affect plant phenotypes
| Autor: | Edouard Severing, Mark G. M. Aarts, Paul Keizer, Jeremy Harbinson, Leo A. J. Willems, Wilco Ligterink, Jeroen van Arkel, Frank Becker, Joost J. B. Keurentjes, Vanesa Calvo-Baltanas, Evangelos Kouklas, David Kramer, Maarten Koornneef, Tom P.J.M. Theeuwen, Raul Wijfjes, Sabine K. Schnabel, Pádraic J. Flood, Fred A. van Eeuwijk, Linda J. Savage, Jos A. Hageman, José M. Gualberto, Roland Mumm, Korbinian Schneeberger, Erik Wijnker, Willem Kruijer |
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| Přispěvatelé: | Department of Plant Developmental Biology, Max Planck Institute for Plant Breeding Research (MPIPZ), CEntre de REcherches en MAthématiques de la DEcision (CEREMADE), Centre National de la Recherche Scientifique (CNRS)-Université Paris Dauphine-PSL, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Mitochondrial DNA Bioinformatics [SDV]Life Sciences [q-bio] Arabidopsis Plant Science Laboratorium voor Erfelijkheidsleer Natural variation Wiskundige en Statistische Methoden - Biometris 01 natural sciences Genome 03 medical and health sciences Bioinformatica Life Science Arabidopsis thaliana [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Laboratorium voor Plantenfysiologie Mathematical and Statistical Methods - Biometris 030304 developmental biology Genetics Organelles 0303 health sciences biology Horticulture & Product Physiology PE&RC biology.organism_classification Phenotype Chloroplast Biometris 030104 developmental biology Variation (linguistics) BIOS Applied Metabolic Systems Epistasis Hybridization Genetic Laboratory of Genetics EPS Ploidy Tuinbouw & Productfysiologie Laboratory of Plant Physiology Genome Plant 010606 plant biology & botany |
| Zdroj: | Nature Plants Nature Plants, Nature Publishing Group, 2020, ⟨10.1038/s41477-019-0575-9⟩ Nature Plants, Nature Publishing Group, 2019 Nature Plants 6 (2020) 1 bioRxiv Nature Plants, 6(1), 13-21 |
| ISSN: | 2055-026X 2055-0278 |
| DOI: | 10.1038/s41477-019-0575-9⟩ |
| Popis: | Assessing the impact of variation in chloroplast and mitochondrial DNA (collectively termed the plasmotype) on plant phenotypes is challenging due to the difficulty in separating their effect from nuclear derived variation (the nucleotype). Haploid inducer lines can be used as efficient plasmotype donors to generate new plasmotype-nucleotype combinations (cybrids)(Ravi et al., 2014). We generated a panel comprising all possible cybrids of seven Arabidopsis thaliana accessions and extensively phenotyped these lines for 1859 phenotypes under stable and fluctuating conditions. We show that natural variation in the plasmotype results in additive as well as epistatic effects across all phenotypic categories. Plasmotypes which induce more additive phenotypic changes also cause more significant epistatic effects, suggesting a possible common basis for both additive and epistatic effects. On average epistatic interactions explained twice as much of the variance in phenotypes as additive plasmotype effects. The impact of plasmotypic variation was also more pronounced under fluctuating and stressful environmental conditions. Thus, the phenotypic impact of variation in plasmotypes is the outcome of multilevel Nucleotype X Plasmotype X Environment interactions and, as such, the plasmotype is likely to serve as a reservoir of variation which is only exposed under certain conditions. The production of cybrids using haploid inducers is a quick and precise method for assessing the phenotypic effects of natural variation in organellar genomes. It will facilitate efficient screening of unique nucleotype-plasmotype combinations to both improve our understanding of natural variation in nucleotype plasmotype interactions and identify favourable combinations to improve plant performance. |
| Databáze: | OpenAIRE |
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